Association between serum level of anti-TPO titer and polymorphisms G1193/C Exon 8 and C2145/T Exon 12 of thyroid peroxidase gene in an Iranian population

Background: Autoimmune thyroid diseases (AITD) are common, with important epidemiological data, including family and twin studies, supporting a strong genetic background on the etiology of AITD.
Objectives: The aim of this study was to assess the relationship between two polymorphisms of Thyroid Peroxidase gene (TPO) and serum level of Anti-TPO titer in an Iranian population.
Patients and Methods: A sample of 184 participants from the Tehran Lipid and Glucose Study was selected as the case (No.=112) and control (No.=72) groups. Inclusion criteria for cases were Anti-TPO and Anti-Tg>100U/L with a history of hypothyroidism. Anti-TPO level in subjects was measured by the ELISA kit. Genomic DNA was extracted using Salting-out/Proteinase K method. Polymorphism detection of Exon 8 and 12 was done using the PCR-RFLP method. The PCR products were incubated with restriction enzymes SacII and BsrI, respectively.
Results: The C allele frequency of C2145/T polymorphism Exon 12 (rs732608) was observed in 71.2% of patients and 28.8% of normal individuals. This allele was significantly associated with increased levels of anti-TPO [(TT, 140±330 pmol/L; vs. CC, 436±380 pmol/L; P < 0.001), (OR: 9.2)]. The G1193/C was not polymorphic and no association between this SNP and the level of serum anti-TPO was found in this study.
Conclusions: We found that the C allele polymorphism in C2145/T exon 12 is associated with the high level of serum anti-TPO and that carriers of this allele are predisposed to disease 9.2 times more than those, who have no C allele. The selected polymorphism of exon 8 has no effect on increased levels of anti-TPO.

2011 Kowsar M.P.Co. All rights reserved.

Please cite this paper as:

Hedayati M, Salehi Jahromi M, Zarif Yeganeh M, Daneshpour MS, Hoghooghi Rad L, Azizi F. Association between serum Level of anti-TPO titer and polymorphisms G1193/C Exon 8 and C2145/T Exon 12 of thyroid peroxidase gene in an Iranian population. Int J Endocrinol Metab. 2010;8(2):64-67.