International Journal of Endocrinology and Metabolism
Research Institute for Endocrine Sciences
Authors
In order to detect nephropathy, measurement of total (24 hrs) urinary albumin or albumin/creatinin ratio in random urine samples is being recommended. But methods of albumin measurement are not available in all laboratories and also cost about 6 times more than that of urinary total protein measurement.
This Study was performed to determine appropriate cut off point in 24 hours urine total protein to diagnose micro- and macroalbuminuria in patients with diabetes mellitus.
In this study, 204 patients with diabetes mellitus type I and II were selected. In collected 24 hours urine from patients, protein and albumin were measured by using Pyrogallol and Immunoturbidimetry methods, respectively.
Normoalbuminuri (albumin < 30 mg/24 hrs urine), microalbuminuri (albumin = 30-300 mg/24 hrs urine), and macroalbuminuri (albumin > 300 mg/24 hrs urine) were detected in 130, 51, and 23 patients, respectively. In 24 hrs urine collections, amounts of protein and albumin were compared to calculate cut off point of exerted protein for nephropathy diagnosis. cut off point of 73 mg/day for urinary total protein had appropriate sensitivity (94.5 %, CI = 91.4 % -97.6 %) and specificity (77.9 %, CI = 72.8 % -82.9 %) for microalbuminuria, while cut off point of 514 mg/day (sensitivity 95.7 %; specificity 98.9 %) was detected for diagnosis macroalbuminuria. Urine protein exertion of 150 mg/day that is currently considered as a normal value in most laboratory kits had a sensitivity of 73.1 % by which 30 % of microalbuminuric cases remained undiagnosed.
Urinary total protein cut-off points of 73 mg/day and 514 mg/day were diagnostic for micro- and macroalbuminuria, respectively.
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Jalali-Khanabadi B. Evaluation of chemical urine dipstick for detection of albuminuria. J Inflamm Dis. 2024;12(4):e155439. doi:
Mohebbi M, Samadi K, Navari N, Ziafati-fahmideh-sani M, Nourihosseini G, et al. The Association Between Serum Magnesium Level and Microalbuminuria in Type 2 Diabetes Mellitus Patients. Nephro-Urol Mon. 2021;13(1):e112373. doi: https://doi.org/10.5812/numonthly.112373
Ansar MM, ShahrokhiRad R, Lebady MK. Risk Factors of Microalbuminuria and Macroalbuminuria in Type 2 Diabetic Patients in North of Iran - Rasht. Nephro-Urol Mon. 2017;9(1):e40031. doi: https://doi.org/10.5812/numonthly.40031
Ziaee A, Shikhileslami H, Sarreshtehdari M, javadi M, Khoeini H. Association of uric acid and albuminuria in patients with type 2 diabetes. J Inflamm Dis. 2024;12(1):e155372. doi:
Iranparvar Alamdari M, Aminisani N, Bashardoost B, Shamshirgaran S, Khodamoradzadeh M, et al. Prevalence and Risk Factors of Microalbuminuria in Type 2 Diabetic Patients in a Diabetic Clinic of Ardabil-Iran. Int J Endocrinol Metab. 2006;4(1):. doi:
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