International Journal of Endocrinology and Metabolism
Mostafa Rezaei-Tavirani1, 
Fariba Fathi2, Fatemeh Darvizeh3, Mohamad Reza Reza Zali4, Mohamad Rostami Rostami Nejad5, 
Kamran Rostami6, Mohsen Tafazzoli2,*, Afsaneh Arefi Arefi oskouie6,*, Seyed AbdolReza AbdolReza Mortazavi-Tabatabaei6Celiac disease (CD) is a disorder associated with body reaction to gluten. After the gluten intake, an immune reaction against the protein occurs and damages villi of small intestine in celiac patients gradually.
The OSC, a filtering method for minimization of inter- and intra-spectrometer variations that influence on data acquisition, was applied to biofluid NMR data of CD patients.
In this study, metabolites of total 56 serum samples from 12 CD patients, 15 CD patients taking gluten-free diet (GFD), and 29 healthy cases were analyzed using nuclear magnetic resonance (NMR) and associated theoretical analysis. Employing ProMetab (version ProMetab_v3_3) software, data obtained from NMR spectra were reduced and orthogonal signal correction (OSC) effect on celiac disease metabonomics before and after the separation by principle component analysis (PCA) was investigated.
The three groups were separated by OSC and findings were analyzed by partial least squares discriminant analysis (PLS-DA) method. Root mean square error of calibration (RMSEc) and correlation coefficient of calibration (Rc) for PLS-DA referred to an efficient group separation filtered by OSC.
The applied leave-one-out cross-validation to PLS-DA method performed along with OSC confirmed validation of data analysis. Finally four metabolites are introduced as CD biomarkers.
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
[email protected] F, Farrokhi-Yekta R, ArefiOskouie A, Eslami M. Survey of potential diagnostic metabolite markers in serum of the rat model of Alzheimer’s disease using nuclear magnatic resonance (1H-NMR) technique. koomesh. 2021;23(1):e153245. doi:
Chashmniam S, Ghafourpour M, Rezaei Farimani A, Gholami A, Nobakht Motlagh Ghoochani BF. Metabolomic Biomarkers in the Diagnosis of Non-Alcoholic Fatty Liver Disease. Hepat Mon. 2019;19(9):e92244. doi: https://doi.org/10.5812/hepatmon.92244
Nobakht Motlagh Ghoochani BF, Aliannejad R, Arefi Oskouie A, fattahi F, Ali Sahakhah H, et al. Nuclear magnetic resonance -based metabolomics analysis of patients exposed to sulfur mustard in different stages using random forest method. koomesh. 2016;17(3):e153909. doi:
Safaei A, Rezaei-Tavirani M, Arefi Oskouie A, Mohebbi SR, Shabani M, et al. Serum Metabolic Profiling of Advanced Cirrhosis Based on HCV. Hepat Mon. 2017;17(3):e44431. doi: https://doi.org/10.5812/hepatmon.44431
sadeghi S, iravani A, arjmand M, vahabi F, eshaghhoseini S, et al. Study of colorectal polyps using 1H Nuclear Magnetic Resonance spectroscopy. koomesh. 2016;17(4):e151192. doi:
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Author(s):
