Comparison of Classic Sweat Test and Crystallization Test in Diagnosis of Cystic Fibrosis

Nima Rezaei; Bahar Pakseresht; Mohammad-Taghi Haghi-Ashtiani; Nooshin Sadjadei; Fatemeh Farahmand; Vajiheh Modaresi

Innovative Journal of Pediatrics

The Scientific Journal of Growth & Development Research Center

Outlines

Abstract
References
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Comparison of Classic Sweat Test and Crystallization Test in Diagnosis of Cystic Fibrosis

Author(s):

Nima Rezaei¹,

Bahar Pakseresht²,

Mohammad-Taghi Haghi-Ashtiani³,

Nooshin Sadjadei⁴,

Fatemeh Farahmand⁵,

Vajiheh Modaresi^6,*

1Molecular Immunology Research Center; and Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran

2Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran

3Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran & Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran

4Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

5Department of Pediatrics, Tehran University of Medical Sciences, Tehran & Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

6Ali-ebne Abitaleb Medical School, Islamic Azad University, Yazd, Iran

Innovative Journal of Pediatrics:Vol. 22, issue 1; 102-106

Published online:Mar 31, 2012

Article type:Research Article

Received:Jan 14, 2011

Accepted:Dec 16, 2011

How to Cite:Rezaei N, Pakseresht B, Haghi-Ashtiani M, Sadjadei N, Farahmand F, et al. Comparison of Classic Sweat Test and Crystallization Test in Diagnosis of Cystic Fibrosis. Inn J Pediatr. 2016;22(1):. doi:

Abstract

Objective: Sweat chloride measurement is considered a standard diagnostic tool for cystic fibrosis (CF). This study was performed to compare sweat chloride values obtained by quantitative pilocarpine iontophoresis (classic test) with sweat crystallization detected by direct observation of a drop of perspiration under light microscopy in patients with and without CF.
Methods: The tests using both techniques were performed simultaneously in patients with and without CF. Cutoff values of ≥60 mmol/L of chloride concentration for the classic sweat test was considered for diagnosis of CF. In crystallization method, observation of typical dendritic forms of salt crystals under light microscopy was interpreted positive.
Findings: Sixty patients suspected to CF (31 males and 29 females) with age range of 9 months to 2 years underwent the sweat test using both techniques. Median sweat chloride values was 26.13+10.85 in group with negative and 72.76+12.78 mmol/L in group with positive sweat test, respectively. All the patients who had positive sweat test in classic method showed typical dendritic forms of salt crystal in sweat crystallization test, which provided the test with 100% sensitivity (95%CI: 93.1-100). Only one of the 31 subjects with negative results for classic sweat test had positive result for crystallization sweat test, which provided the test with 96.7% specificity (95%CI: 92.9-100). Time spent to perform the crystallization test was significantly shorter than the classic method whereas its cost was also lower than the second method.
Conclusion: There was a good correspondence between two studied methods of sweat test. These results suggested the sweat crystallization test as an alternative test for detecting CF disease with high sensitivity and specificity.

Keywords

Cystic fibrosis

Diagnosis

Sweat chloride

Sweat crystallization

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References

1.
References are available in PDF.

Comparative Study of Urinary Minerals and Their Effect on Stone Formation in Two Groups of Cystic Fibrosis (CF) and Healthy Children

Alireza Niknahad,

Lobat Shahkar,

Farshid Kompani,

Maryam Montazeri,

Ali Ahani Azar

Niknahad A, Shahkar L, Kompani F, Montazeri M, Ahani Azar A. Comparative Study of Urinary Minerals and Their Effect on Stone Formation in Two Groups of Cystic Fibrosis (CF) and Healthy Children. Nephro-Urol Mon. 2020;12(3):e105530. doi: https://doi.org/10.5812/numonthly.105530

11

Mar

2018

The Acute Effect of Inhaled NaCl 5%, Per CF TRUST Protocol, on Spirometry Indices in Patients Over Six Years with Cystic Fibrosis

Seyed-Ahmad Tabatabaei,

Gholamreza Panahandeh,

Ghamartaj Khanbabaei,

Saeid Sadr

Tabatabaei S, Panahandeh G, Khanbabaei G, Sadr S. The Acute Effect of Inhaled NaCl 5%, Per CF TRUST Protocol, on Spirometry Indices in Patients Over Six Years with Cystic Fibrosis. Arch Pediatr Infect Dis. 2018;6(2):e12063. doi: https://doi.org/10.5812/pedinfect.12063

12

Sep

2014

Dermatoglyphic Patterns in Cystic Fibrosis Children

Atefeh Ezzati,

Fereshteh Batoei,

Seyed-Ali Jafari,

Mohammad-Ali Kiyani,

Naser Mahdavi-Shahri,

Hamid Ahanchian

,et al.

Ezzati A, Batoei F, Jafari S, Kiyani M, Mahdavi-Shahri N, et al. Dermatoglyphic Patterns in Cystic Fibrosis Children. Inn J Pediatr. 2014;24(5):. doi:

18

Apr

2015

A Case of Cystic Fibrosis With a Rare Mutation (3849 + 10 kbC > T) and Normal Sweat Chloride Levels

Taha Resid Ozdemir,

Ali Kanik

Ozdemir TR, Kanik A. A Case of Cystic Fibrosis With a Rare Mutation (3849 + 10 kbC > T) and Normal Sweat Chloride Levels. Inn J Pediatr. 2015;25(2):e369. doi: https://doi.org/10.5812/ijp.369

10

Jun

2019

The Efficacy of Nebulized Soluble Mannitol and Comparison with 5% Hypertonic Saline on Pulmonary Function of Children with Cystic Fibrosis

Saeed Sadr,

Masood Kiani,

Mohammad Rezaei,

Ghamartaj Khanbabaee,

Seyed Ahmad Tabatabaee,

Amirhossein Hosseini

Sadr S, Kiani M, Rezaei M, Khanbabaee G, Tabatabaee SA, et al. The Efficacy of Nebulized Soluble Mannitol and Comparison with 5% Hypertonic Saline on Pulmonary Function of Children with Cystic Fibrosis. J Compr Ped. 2019;10(3):e85616. doi: https://doi.org/10.5812/compreped.85616

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Author(s):

Nima Rezaei:[PubMed][Scholar]
Bahar Pakseresht:[PubMed][Scholar]
Mohammad-Taghi Haghi-Ashtiani:[PubMed][Scholar]
Nooshin Sadjadei:[PubMed][Scholar]
Fatemeh Farahmand:[PubMed][Scholar]
Vajiheh Modaresi:[PubMed][Scholar]

The Scientific Journal of Growth & Development Research Center

Outlines

Comparison of Classic Sweat Test and Crystallization Test in Diagnosis of Cystic Fibrosis

Abstract

Fulltext

References

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