Fulltext
The full text of this article is available on PDF.
Journal of Clinical Research in Paramedical Sciences
The Official Journal of Paramedical School, KUMS
e-ISSN 2345-3338
p-ISSN 2322-1593
Image Credit:J Clin Res Paramed Sci
Outline
Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah
Authors
1. Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Department of Microbiology, Azad Univerisy of Sanandaj, Sanandaj, Iran
*Corresponding Author: Corresponding author: Alisha Akya, Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran, E-mail: Email: akya359@yahoo.com
Published online:Aug 13, 2015
Article type:Research Article
Received:Feb 14, 2015
Accepted:Jun 18, 2015
How to Cite: Salimi A, Akya A, Haidari E. Prevalence of 𝛽-lactamases genes of IMP, SHV and PER in Pseudomonas aeruginosa isolated from hospitals in Kermanshah. J Clin Res Paramed Sci. 2015;4(2):e82001. doi:
Abstract
Background: Pseudomonas aeruginosa is an opportunistic pathogen associated with nosocomial infections. The emergence and dissemination of metallo-𝛽-lactamases (MBLs) and extendedspectrum-𝛽-lactamases (ESBLs) have contributed to the high rate of
resistance among the p. aeruginosa isolates. This study investigated the prevalence of IMP, SHV and PER genes in p. aeruginosa isolates.
Methods: Antibiotic susceptibility testing was performed using the minimal inhibitory concentrations (MIC). Then the polymerase chain reaction (PCR) was used for the detection of 3 genes encoding IMP, SHV and PER.
Results: Of the 60 isolates tested, 38 (63.3%) were resistant to piperacilin, 42 (70%) to ceftazidime, 44(73.3%) to imipenem and 45 (75%) to cefepime. Five (8.3%) and 8(13.3%) of the isolates had PER and SHV genes by PCR results. In this study IMP gene was not found.
Conclusion: According to this study, P. aeruginosa isolates were highly resistant to antibiotics. Therefore the susceptibility of isolates should be tested before treatment. Among the three β-lactamases genes studied the highest prevalence was related to SHV gene. Given the clinical significance of MBLs and ESBLs producing isolates, identification of these organisms is essential in the hospitals in order to get a better therapeutic response and control of bacterial dissemination.
Highlights
References
- 1.The References of this article is available on PDF.
Copyright
© 2015, Journal of Clinical Research in Paramedical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
Similar Articles
1
Jan
2014
Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran
Mohammad Yousef Alikhani,
Zahra Karimi Tabar,
Fatemeh Mihani,
Enayat Kalantar,
Pegman Karami,
Mahnaz Sadeghi
,et al.Alikhani MY, Karimi Tabar Z, Mihani F, Kalantar E, Karami P, et al. Antimicrobial Resistance Patterns and Prevalence of blaPER-1 and blaVEB-1 Genes Among ESBL-producing Pseudomonas aeruginosa Isolates in West of Iran. Jundishapur J Microbiol. 2014;7(1):e8888. doi: https://doi.org/10.5812/jjm.8888
22
Sep
2015
Detection and Genetic Characterization of Metallo-β-Lactamase IMP-1 and VIM-2 in Pseudomonas aeruginosa Strains From Different Hospitals in Kermanshah, Iran
Ramin Abiri,
Pantea Mohammadi,
Navid Shavani,
Mansour Rezaei
Abiri R, Mohammadi P, Shavani N, Rezaei M. Detection and Genetic Characterization of Metallo-β-Lactamase IMP-1 and VIM-2 in Pseudomonas aeruginosa Strains From Different Hospitals in Kermanshah, Iran. Jundishapur J Microbiol. 2015;8(9):e22582. doi: https://doi.org/10.5812/jjm.22582
20
Jun
2023
Evaluation of β-lactamases and Molecular Typing of Pseudomonas aeruginosa Clinical Strains Isolated from Hospitalized Children in Tehran, Iran
Hossein Goudarzi,
Narjess Bostanghadiri,
Zahra Riahi Rad,
Zohreh Riahi Rad,
Javad Yasbolaghi Sharahi
Goudarzi H, Bostanghadiri N, Riahi Rad Z, Riahi Rad Z, Yasbolaghi Sharahi J. Evaluation of β-lactamases and Molecular Typing of Pseudomonas aeruginosa Clinical Strains Isolated from Hospitalized Children in Tehran, Iran. Arch Clin Infect Dis. 2023;18(2):e134837. doi: https://doi.org/10.5812/archcid-134837
8
Dec
2014
Frequency of PER, VEB, SHV, TEM and CTX-M Genes in Resistant Strains of Pseudomonas aeruginosa Producing Extended Spectrum β-Lactamases
Mohmmad Bokaeian,
Shahram Shahraki Zahedani,
Morteza Soltanian Bajgiran,
Alireza Ansari Moghaddam
Bokaeian M, Shahraki Zahedani S, Soltanian Bajgiran M, Ansari Moghaddam A. Frequency of PER, VEB, SHV, TEM and CTX-M Genes in Resistant Strains of Pseudomonas aeruginosa Producing Extended Spectrum β-Lactamases. Jundishapur J Microbiol. 2015;8(1):e13783. doi: https://doi.org/10.5812/jjm.13783
13
Sep
2021
Molecular Detection of Carbapenemases and Extended-Spectrum β-Lactamases-Encoding Genes in Clinical Isolates of Pseudomonas aeruginosa in Iran
Esmaeil Rahimi,
Ali Asgari,
Taher Azimi,
Saeed Soleiman-Meigooni
Rahimi E, Asgari A, Azimi T, Soleiman-Meigooni S. Molecular Detection of Carbapenemases and Extended-Spectrum β-Lactamases-Encoding Genes in Clinical Isolates of Pseudomonas aeruginosa in Iran. Jundishapur J Microbiol. 2021;14(7):e115977. doi: https://doi.org/10.5812/jjm.115977
Cited by
- Scopus by DOI: 0
Last Update: 1 week ago
- Scopus by Title: 0
Last Update: 1 week ago
- Scopus by Title (Ref): 1
Last Update: 1 week ago
- CrossRef: 0
Last Update: 4 hours ago
- OpenAlex: 0
Last Update: 7 hours ago
- OpenCitations: 0
Last Update: 5 days ago
Comments
Number of Comments:0