Journal of Inflammatory Diseases
The Official Journal of Qazvin University of Medical Sciences
Assessment of mutual impact of different sodium citrate concentrations and minimum specimen volume on the result of routine coagulation tests
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Abstract
Abstract Background: Routine coagulation assays including prothrombine time [PT] and activated partial thromboplastine time [aPTT] are influenced by two independent parameters comprising sample volume and tri-sodium citrate concentration. Objective: To evaluate minimum specimen volume requirements for routine coagulation testing with dependence on citrate concentration. Methods: An experimental study was performed on samples obtained from randomized healthy persons and patients receiving oral anticoagulant therapy. The tubes (3.2% and 3.8% sodium citrate) were filled to varying total sample volumes ranging from 3.0 to 5.0 mL and results of routine coagulation tests were compared. Findings: By using 3.8% citrate, there is a statistically significant difference in the results of PT and aPTT assays in the sample less than 80% and 90% filled compared with those that are 100% filled. There is no significant difference in PT results from a 3.2% citrate tube between fill volume of 60% and 100% as well as 70% and 100% for aPTT. Conclusion: Underfilling may significantly affect the aPTT and PT, resulting in artifactual prolongation of results, particularly in samples drawn into 3.8% citrate. This study supports the recommendations to use 3.2% citrate, because 60% of the optimum filled for PT and 70% for aPTT are acceptable.
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© 2024, Journal of Inflammatory Diseases. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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