Journal of Inflammatory Diseases
The Official Journal of Qazvin University of Medical Sciences
Genetic variations of OprD porin protein in imipenem resistant clinical isolates of Pseudomonas aeruginosa in burn patients
Authors
Abstract
Background: Drug resistance is one of the important threats in uncontrolled infections by Pseudomonas aeruginosa, an opportunistic nosocomial pathogen, in burn patients. The presence of OprD porin protein in the bacterial cell wall is one of the mechanisms for resistance against hydrophilic drugs in this bacterium. Objective: The aim of this study was to evaluate genetic sequence rearrangements of OprD gene in imipenem resistant clinical isolates of pseudomonas aeruginosa in burn patients. Methods: This cross sectional study was performed in Ghotbeddin Shirazi Hospital from October 2013 to February 2015. A total of 253 wound samples were evaluated for Pseudomonas aeruginosa. All isolates were evaluated using specific sequencing of the target region. Genetic sequence rearrangements were compared with the sensitivity pattern of the isolates to the imipenem. Findings: Pseudomonas aeruginosa was found in 22% of the samples in Shiraz burn center. More than 90% of the isolates were multi drug resistant while only 25% were sensitive to imipenem. More than 80% of the imipenem resistant isolates had rearrangement in the gene associated with OprD protein. Conclusion: With regards to the results, it seems that Pseudomonas aeruginosa, as a prevalent microorganism in burn wounds, has rearrangement in the gene associated with OprD porin protein. This rearrangement may play a role in drug resistance of the Pseudomonas aeruginosa isolates in hospitalized patients.
Highlights
Copyright
© 2024, Journal of Inflammatory Diseases. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
Similar Articles
Examining the Frequency of Carbapenem Resistance Genes and Its Relationship with Different Classes of Integrons Including Classes I and II in Pseudomonas aeruginosa Isolates of Burn Patients
Asghari Gharakhyli E, Norollahi SE, Sedigh Ebrahim-Saraie H, Babaei K, Hasannejad-Bibalan M, et al. Examining the Frequency of Carbapenem Resistance Genes and Its Relationship with Different Classes of Integrons Including Classes I and II in Pseudomonas aeruginosa Isolates of Burn Patients. Jundishapur J Microbiol. 2023;16(11):e140912. doi: https://doi.org/10.5812/jjm-140912
The Prevalence of Aminoglycoside-Modifying Enzyme Genes Among Pseudomonas aeruginosa Strains Isolated From Burn Patients
Kashfi M, Hashemi A, Eslami G, Sadredin Amin M, Tarashi S, et al. The Prevalence of Aminoglycoside-Modifying Enzyme Genes Among Pseudomonas aeruginosa Strains Isolated From Burn Patients. Arch Clin Infect Dis. 2017;12(1):e40896. doi: https://doi.org/10.5812/archcid.40896
Class A and D Extended-Spectrum β-Lactamases in Imipenem Resistant Pseudomonas aeruginosa Isolated From Burn Patients in Iran
Pakbaten Toupkanlou S, Najar Peerayeh S, Pirhajati Mahabadi R. Class A and D Extended-Spectrum β-Lactamases in Imipenem Resistant Pseudomonas aeruginosa Isolated From Burn Patients in Iran. Jundishapur J Microbiol. 2015;8(8):e59888. doi: https://doi.org/10.5812/jjm.18352v2
Phenotypic and Molecular Detection of Metallo-Beta-Lactamase Genes Among Imipenem Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Strains Isolated From Patients with Burn Injuries
Tarashi S, Goudarzi H, Erfanimanesh S, Pormohammad A, Hashemi A. Phenotypic and Molecular Detection of Metallo-Beta-Lactamase Genes Among Imipenem Resistant Pseudomonas aeruginosa and Acinetobacter baumannii Strains Isolated From Patients with Burn Injuries. Arch Clin Infect Dis. 2016;11(4):e39036. doi: https://doi.org/10.5812/archcid.39036
Assessment of Carbapenem Susceptibility and Multidrug-Resistance in Pseudomonas aeruginosa Burn Isolates in Tehran
Moazami-Goudarzi S, Eftekhar F. Assessment of Carbapenem Susceptibility and Multidrug-Resistance in Pseudomonas aeruginosa Burn Isolates in Tehran. Jundishapur J Microbiol. 2013;6(2):162-165. doi: https://doi.org/10.5812/jjm.5036
- Scopus by DOI: 0
Last Update: 1 week ago
- Scopus by Title: 0
Last Update: 1 week ago
- Scopus by Title (Ref): 0
Last Update: 1 week ago
- CrossRef: 0
Last Update: 5 days ago
- OpenAlex: 0
Last Update: 39 minutes ago
- OpenCitations: 0
Last Update: 3 days ago