A study on genotypes of hepatitis B virus among hemodialysis patients in Khuzestan province

Niloofar Neisi; Manochehr Makvandi; Ali Reza Samarbaf-Zadeh

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

Current Issue All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

A study on genotypes of hepatitis B virus among hemodialysis patients in Khuzestan province

Author(s):

Niloofar Neisi

^1,*,

Manochehr Makvandi²,

Ali Reza Samarbaf-Zadeh²

1Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, [email protected], Iran

2Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Iran

Jundishapur Journal of Microbiology:Vol. 4, issue 2

Published online:Apr 30, 2011

Article type:Research Article

How to Cite:Neisi N, Makvandi M, Samarbaf-Zadeh AR. A study on genotypes of hepatitis B virus among hemodialysis patients in Khuzestan province. Jundishapur J Microbiol. 2011;4(2):. doi:

Abstract

Introduction and objective: Hepatitis B virus (HBV) is a member of Hepadnaviridae and a major causative agent of chronic and acute hepatitis, liver cirrhosis and hepatocellular carcinoma. Genotype determination of HBV is based on PCR-RFLP and sequencing of genome of the virus. The genotype formation is mainly due to mutations of HBV precore, S, and YMDD (tyrosine-methionine-aspartate-aspartate motif in the C domain of the HBV DNA polymerase gene) genome area. Moreover, some of the mutant HBV remains undetectable by serological tests (occult hepatitis). Since the genotypes of HBV and occult hepatitis B has not been studied in our area, this study was conducted to determine both occult hepatitis B infection and genotypes among hemodialysis patients.

Materials and methods: Two hundred and fifty hemodialysis patients were selected in this study. The sera of the patients were collected and the extracted DNA was used as template of PCR to amplify a 479bp fragment of the viral genome. The PCR products were digested by Ava2 and Mbo1 restriction enzymes. Based on RFLP patterns, the genotypes were determined. The HBV markers including; HBV surface antigen (HBsAg), hepatitis Bc antibody (HBcIgG), hepatitis B virus e antigen (HBeAg) and hepatitis B virus e antibody (HBeAb) were carried out for all the patients by ELISA test.

Results: Fifty (20%) out of 250 sera showed positive HBV by PCR. Out of the 50 positive cases for HBV, 46(92%) belonged to genotype D2 and 2(4%) cases of them were B6 genotype. Ten cases were positive for HBV by PCR test but negative by ELISA test (4% occult hepatitis).

Conclusion: Prevalence of HBV infection was high among the dialysis patients (20%), and occult hepatitis B was 4% in these patients. The dominant genotype of HBV was D2 (92%) followed by genotypes B6 (4%) in hemodialysis patients.

Keywords

Hepatitis B virus (HBV)

Hemodialysis

Genotyping

RFLP

Full Text

Full text is available in PDF

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 6 days ago
Scopus by Title: 14
Last Update: 6 days ago
Scopus by Title (Ref): 15
Last Update: 6 days ago
CrossRef: 0
Last Update: 33 minutes ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Niloofar Neisi:[PubMed][Scholar]
Manochehr Makvandi:[PubMed][Scholar]
Ali Reza Samarbaf-Zadeh:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

Outlines

A study on genotypes of hepatitis B virus among hemodialysis patients in Khuzestan province

Abstract

Full Text

Last Update: 6 days ago

Last Update: 6 days ago

Last Update: 6 days ago

Last Update: 33 minutes ago