A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran

Hamid Abdollahi; Mohammad Javad Zahedi; Sodief Darvish Moghadam; Mehdi Hayatbakhsh Abasi

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran

Author(s):

Hamid Abdollahi^1,*,

Mohammad Javad Zahedi²,

Sodief Darvish Moghadam²,

Mehdi Hayatbakhsh Abasi²

1Department of Medical Microbiology, Virology and Immunology, Kerman University of Medical Sciences, [email protected], Iran

2Department of Gastroenterology, Kerman University of Medical Sciences, Iran

Jundishapur Journal of Microbiology:Vol. 4, issue 2

Published online:Apr 30, 2011

Article type:Research Article

Received:Jan 01, 2011

Accepted:Jan 20, 2011

How to Cite:Abdollahi H, Javad Zahedi M, Darvish Moghadam S, Hayatbakhsh Abasi M. A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran. Jundishapur J Microbiol. 2011;4(2):. doi:

Abstract

Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori.
Materials and methods: We tested 63 H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti-H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other.
Results: From 35 resistant H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates.
Conclusion: According to rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved.

Keywords

Helicobacter pylori

Metronidazole resistant

rdxA deletion

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Author(s):

Hamid Abdollahi:[PubMed][Scholar]
Mohammad Javad Zahedi:[PubMed][Scholar]
Sodief Darvish Moghadam:[PubMed][Scholar]
Mehdi Hayatbakhsh Abasi:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

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A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran

Abstract

Full Text

Last Update: 6 days ago

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Last Update: 51 minutes ago