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Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
e-ISSN 2008-4161
p-ISSN 2008-3645
A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran
Authors
1Department of Medical Microbiology, Virology and Immunology, Kerman University of Medical Sciences, mohammad1318@yahoo.com, Iran
2Department of Gastroenterology, Kerman University of Medical Sciences, Iran
*Corresponding Author: Department of Medical Microbiology, Virology and Immunology, Kerman University of Medical Sciences, mohammad1318@yahoo.com, Iran. Tel: +98341 3221665. Fax: +98341 3221665. Email: mohammad1318@yahoo.com
Jundishapur Journal of Microbiology:Vol. 4, issue 2
Published online:Apr 30, 2011
Article type:Research Article
Received:Jan 01, 2011
Accepted:Jan 20, 2011
How to Cite:Abdollahi H, Javad Zahedi M, Darvish Moghadam S, Hayatbakhsh Abasi M. A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran. Jundishapur J Microbiol. 2011;4(2):. doi:
Abstract
Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori.
Materials and methods: We tested 63 H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti-H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other.
Results: From 35 resistant H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates.
Conclusion: According to rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved.
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Copyright
© 2011, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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