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Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

e-ISSN 2008-4161
p-ISSN 2008-3645
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Cover image of the article: Combined Application of Microbial Cellulose and Papaver macrostomum Extract on Bedsore Microorganisms

Image Credit:Jundishapur J Microbiol

Outline

https://doi.org/10.5812/jjm.4807

Combined Application of Microbial Cellulose and Papaver macrostomum Extract on Bedsore Microorganisms

Authors

Anita Khanafari1,*, Golnaz Yaghoub Nezhad Zangeneh1, Fariba Sharifnia2
1Microbiology Department, North Tehran Branch, Islamic Azad University, Tehran, IR Iran
2Biology Department, North Tehran Branch, Islamic Azad University, Tehran, IR Iran
*Corresponding Author: Microbiology Department, North Tehran Branch, Islamic Azad University, Tehran, IR Iran. Tel: +98-2122082141, khanafari_a@yahoo.com. Email: khanafari_a@yahoo.com
Jundishapur Journal of Microbiology:Vol. 6, issue 3; 220-5
Published online:Apr 30, 2013
Article type:Research Article
Received:Mar 12, 2012
Accepted:Jul 02, 2012
How to Cite:Khanafari A, Yaghoub Nezhad Zangeneh G, Sharifnia F. Combined Application of Microbial Cellulose and Papaver macrostomum Extract on Bedsore Microorganisms. Jundishapur J Microbiol. 2013;6(3):. doi: https://doi.org/10.5812/jjm.4807

Abstract

Background:

Bedsore is one of the major problems in all the societies as patients are confined to bed. Due to antibiotic resistant strains being a significant obstacle for cure, many plants and herbs are being used by researchers as medicinal compounds.

Objectives:

The investigation of synergistic effect of cellulose biopolymer and Papaver macrostomum extract on bedsores bacterial community.

Materials and Methods:

Acetobacter xylinum PTTC 1734 was cultured in Schramm-Hestrin (SH) medium and incubated at 30C for 24-48 hours. NaOH treatment and absolute ethanol were used to extract cellulose biopolymer and plant antimicrobial substance, respectively. The Biopolymer structure was scanned by a Scanning electron microscope (SEM). Antimicrobial activities, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of these extracts were all determined separately. The effective concentration of each extract's alone, combined, and synergistic effects were evaluated. Biopolymer absorption efficiency was assayed as the absorbent bed.

Results:

Pesudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus were the dominate bacteria isolated from bedsore samples. Antimicrobial effects of cellulose, P. macrostomum extract, and the combination of both were determined on the isolated bacteria as 1, 10, and 15 mm respectively. 100-1000?l/mL of flower ethanol extract concentrations of P. macrostomum indicated the maximum effect on mixed bedsore's bacteria rather than leaf and mixed extraction. Concentrations 500-1000?l/mL decreased the bacterial bedsore's growth and completely inhibited it. 3.5g/L of cellulose biopolymer was obtained from A. xylinum broth culture medium. Scanning electron microscopy analysis confirmed the branched structure of this polymer. Cellulose absorption efficiency was evaluated to be 14.5ml/g in this investigation. Because of high-absorbance of bio-cellulose, combined plant extraction with this biopolymer caused a decrease in the growth of bedsore microorganisms with the minimum extract concentration, 100?l.

Conclusions:

Combination of bio-cellulose and P. macrostomum flower ethanol extract can be used for patients who suffer from bedsore lesions in concentration 0.1% of MBCs. Furthermore, clinical studies are needed to confirm the efficiency of in vivo application.

Highlights

KeywordsBedsores<italic>Acetobacter xylinum</italic>Cellulose Biopolymer<italic>Papaver macrostomum</italic>Antimicrobial Effect

Full Text

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Copyright

© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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