Full Text
Full text is available in PDF
Background:
One disadvantage of expressing heterologous proteins in
Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Using Rice Flour for Purification of Maltose Binding Fusion Proteins Expressed in Escherichia coli
Author(s):
1Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
2Department of Pathobiology, School of Veterinary Medicine, University of Lorestan, Khorram Abad, IR Iran
Published online:Apr 30, 2013
Article type:Research Article
Received:Mar 17, 2012
Accepted:Jun 30, 2012
How to Cite:Neissi A, Seyfi Abad Shapouri M, Ghorbanpoor Najafabadi M, Jaydary A. Using Rice Flour for Purification of Maltose Binding Fusion Proteins Expressed in Escherichia coli. Jundishapur J Microbiol. 2013;6(3):. doi: https://doi.org/10.5812/jjm.4926
Abstract
Objectives:
The purpose of this study was to investigate the use of rice flour, a natural source of amylose, for purification of MBP fusion proteins.
Materials and Methods:
MBP and a fusion protein of MBP and avian influenza virus nucleoprotein (MBP-NP) were expressed in
Results:
The results indicated that MBP and MBP-NP, both were successfully purified by rice flour.
Conclusions:
Rice flour can be used for purification of MBP fusion proteins. Although the efficiency of purification by rice flour is less than amylose resin, however, the yield is sufficient to obtain a quantity of protein required for research purposes.
Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
1
Sep
2013
Optimization and Efficient Purification in Production of Brucella melitensis Recombinant HSP and TF Proteins With Low Endotoxin Contents
Amir Ghasemi,
Mohammad Hossein Salari,
Mohammad Reza Pourmand,
Amir Hassan Zarnani,
Hojat Ahmadi,
Mohamad Hassan Shirazi
,et al.Ghasemi A, Salari MH, Pourmand MR, Zarnani AH, Ahmadi H, et al. Optimization and Efficient Purification in Production of Brucella melitensis Recombinant HSP and TF Proteins With Low Endotoxin Contents. Jundishapur J Microbiol. 2013;6(7):e6875. doi: https://doi.org/10.5812/jjm.6875
18
Nov
2018
Cloning, Expression and Purification of a Novel Fusion Protein Composed of Flagellin and NS5B of Hepatitis C Virus in Escherichia coli Host
Amir Atapour,
Zohreh Mostafavi-Pour,
Pooneh Mokarram,
Shiva Mohammadi
Atapour A, Mostafavi-Pour Z, Mokarram P, Mohammadi S. Cloning, Expression and Purification of a Novel Fusion Protein Composed of Flagellin and NS5B of Hepatitis C Virus in Escherichia coli Host. Shiraz E-Med J. 2018;20(1):e79898. doi: https://doi.org/10.5812/semj.79898
18
Apr
2015
Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae
Minoo Tavakoli,
Saeed Bouzari,
Seyed Davar Siadat,
Shahin Najar Peerayeh,
Anis Jafari
Tavakoli M, Bouzari S, Siadat SD, Najar Peerayeh S, Jafari A. Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae. Jundishapur J Microbiol. 2015;8(4):e16377. doi: https://doi.org/10.5812/jjm.8(4)2015.16377
29
Aug
2015
Molecular Cloning, Expression and Purification of Truncated hpd Fragment of Haemophilus influenzae in Escherichia coli
Ava Behrouzi,
Saeid Bouzari,
Seyed Davar Siadat,
Anis Jafari,
Shiva Irani
Behrouzi A, Bouzari S, Siadat SD, Jafari A, Irani S. Molecular Cloning, Expression and Purification of Truncated hpd Fragment of Haemophilus influenzae in Escherichia coli. Jundishapur J Microbiol. 2015;8(8):e23218. doi: https://doi.org/10.5812/jjm.23218
2
Jul
2018
Engineering, Cloning and Expression of DNA Sequence Coding of OMP31 Epitope of Brucella melitensis linked to IL-2 in Escherichia coli
Narges Nazifi,
Mojtaba Tahmoorespur,
Mohammad Hadi Sekhavati,
Alireza Haghparast,
Mohammad Ali Behroozikhah
Nazifi N, Tahmoorespur M, Sekhavati MH, Haghparast A, Behroozikhah MA. Engineering, Cloning and Expression of DNA Sequence Coding of OMP31 Epitope of Brucella melitensis linked to IL-2 in Escherichia coli. Int J Infect. 2018;5(3):e68974. doi: https://doi.org/10.5812/iji.68974
Crossmark
Checking
Comments
Number of Comments:0Cited by
- Scopus by DOI: 2
Last Update: 1 month ago
- Scopus by Title: 2
Last Update: 1 month ago
- Scopus by Title (Ref): 2
Last Update: 1 month ago
- CrossRef: 0
Last Update: 1 day ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCCÂ
Search Relations
Author(s):
Related Articles