Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Authors
Listeria monocytogenes causes listeriosis characterized by encephalitis, septicaemia, and abortion or stillbirth. Its traditional diagnosis is based on serological responses, whileseveral molecular methods have been developed for safer and more rapid, sensitive, and accurate detection.The epidemiology, prevalence, shedding routes, and antibiotic resistance properties of L. monocytogenes are essentially unknown in various animals species.
The present study was performed to study the shedding routes, and antibiotic resistance properties of L. monocytogenes isolated from milk, feces, urine, and vaginal secretion of bovine, ovine, caprine, buffalo, and camel in Iran.
A total of 596 milk, 619 feces, 443 vaginal swab, and 522 urine samples were collected from various animal species. Samples were examined by culture, conventional and real-time PCR for evaluating the presence of L. monocytogenes. Finally antimicrobial resistance properties were studied using the simple disc diffusion method.
The culture method showed that 186 of 2180 samples (8.53%) had positive results for L monocytogenes. In total,61 (10.23%) milk, 40 (6.46%) feces, 43 (9.7%) vaginal swab, and 48 (9.19%) urine samples had positive results for L monocytogenes using conventional PCR. After the Light Cycler real-time PCR it was recognized that 69 (11.57%) milk, 48 (7.75%) feces, 53 (11.96%) vaginal swab and 57 (10.91%) urine samples hadpositive results for the presence of
Shedding of L. monocytogenes in different animal species, and different samples are different. Due to antibiotic resistance, especially in L. monocytogenes, veterinarians should pay more attention to prescribe antibiotics. We recommend using the real-time PCR for safe, sensitive, and rapid detection of L. monocytogenes in clinical samples, and using the disk diffusion methods to prescribe suitable antibiotics.
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Tahmasby H. Molecular and biochemical evaluation of ovine carcasses contamination to Listeria monocytogenes in Shahrekord. Jentashapir J Cell Mol Biol. 2012;3(3):. doi:
Zarei M, Basiri N, Jamnejad A, Eskandari MH. Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR. Jundishapur J Microbiol. 2013;6(8):e7244. doi: https://doi.org/10.5812/jjm.7244
Davati N, Tabatabaee Yazdi F, Zibaee S, Shahidi F, Edalatian MR. Study of Lactic Acid Bacteria Community From Raw Milk of Iranian One Humped Camel and Evaluation of Their Probiotic Properties. Jundishapur J Microbiol. 2015;8(5):e16750. doi: https://doi.org/10.5812/jjm.8(5)2015.16750
Ghazaei C. Evaluation of prf A Gene in Listeria monocytogenes Isolated from Dairy. J Clin Res Paramed Sci. 2024;13(2):e145845. doi: https://doi.org/10.5812/jcrps-145845
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