Fulltext
Full text is available in PDF
Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Restriction Fragment Length Polymorphism Genotyping of Human Staphylococcus aureus Isolates From Two Hospitals in Urmia Region of Iran Using the coa Gene
Author(s):
1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, IR Iran
*Corresponding Author: Malahat Ahmadi, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, P. O. Box 1177, Urmia, IR Iran; Tel: +98-4412972649, Fax: +98-4412771926, E-mail: Email: [email protected]
Published online:Apr 30, 2012
Article type:Research Article
Received:Jul 01, 2011
Accepted:Nov 01, 2011
How to Cite:Talebi-Satlou R, Ahmadi M, Dastmalchi Saei H. Restriction Fragment Length Polymorphism Genotyping of Human Staphylococcus aureus Isolates From Two Hospitals in Urmia Region of Iran Using the coa Gene. Jundishapur J Microbiol. 2012;5(2):. doi: https://doi.org/10.5812/jjm.3522
Abstract
Background: Staphylococcus aureus has become an emerging public health concern. Markers that differentiate tissue-specific lineages are needed to trace the sources of strains.
Objectives: The aims of this study were to determine the genotypic characteristics of
S. aureus isolates that are associated with skin and urinary tract infections using polymorphisms in the coagulase gene.
Materials and Methods: Coagulase gene variants among 26 S. aureus isolates from human infected skin (n = 10) and urine (n = 16) samples were investigated by amplification of the repeat units encoding the hypervariable region of the coagulase gene. The amplicons ranged from 490-790 bp and were subjected to restriction fragment length polymorphism (RFLp) analysis with HaeIII.
Results: In total, 6 distinct RFLp banding patterns were observed, designated C1-C6.The C1 pattern predominated in skin and urine isolates. Notably, the C3, C5, and C6 patterns were present in isolates from urine, whereas the C2 and C4 genotypes were preferentially detected in skin sample isolates.
Conclusions: These data demonstrate the widespread prevalence of certain genotypes and tissue-specific tendency of other genotypes, suggesting the existence of lineage- and tissue-specific genes that mediate the development of tissue-specific pathogenicities of S. aureus isolates.
Copyright © 2012 Kowsar Corp. All rights reserved.
Copyright
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
2
Jan
2016
Genotyping of coa and aroA Genes of Methicillin-Resistant Staphylococcus aureus Strains Isolated From Nasal Samples in Western Iran
Parviz Mohajeri,
Samira Azizkhani,
Abbas Farahani,
Baharak Norozi
Mohajeri P, Azizkhani S, Farahani A, Norozi B. Genotyping of coa and aroA Genes of Methicillin-Resistant Staphylococcus aureus Strains Isolated From Nasal Samples in Western Iran. Jundishapur J Microbiol. 2016;9(1):e26460. doi: https://doi.org/10.5812/jjm.26460
17
May
2017
Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran
Seyed Asghar Havaei,
Mehrdad Halaji,
Sinisa Vidovic,
Jo-Anne R. Dillon,
Mohsen Karbalaei,
Fahimeh Ghanbari
,et al.Havaei SA, Halaji M, Vidovic S, R. Dillon J, Karbalaei M, et al. Prevalence and Genotyping of Methicillin-Resistant and - Susceptible Staphylococcus aureus Strains Isolated from Patients in a University Hospital, Isfahan, Iran. Jundishapur J Microbiol. 2017;10(5):e13571. doi: https://doi.org/10.5812/jjm.13571
9
Mar
2016
Molecular Typing of Staphylococcus aureus Isolated From Clinical Specimens During an Eight-Year Period (2005 - 2012) in Tabriz, Iran
Mohammad Ahangarzadeh Rezaee,
Seyed Foad Mirkarimi,
Alka Hasani,
Vajihe Sheikhalizadeh,
Mohammad Hossein Soroush,
Babak Abdinia
Ahangarzadeh Rezaee M, Mirkarimi SF, Hasani A, Sheikhalizadeh V, Soroush MH, et al. Molecular Typing of Staphylococcus aureus Isolated From Clinical Specimens During an Eight-Year Period (2005 - 2012) in Tabriz, Iran. Arch Pediatr Infect Dis. 2016;4(2):e35563. doi: https://doi.org/10.5812/pedinfect.35563
1
Aug
2013
Evaluation of Protein A Gene Tandem Repeat Polymorphism of Staphylococcus aureus Isolated From Bovine Mastitis in Tabriz
Mohammad Ali Hosseinpour Feizi,
Gholamreza Zarrini,
Mehdi Tahmasebi
Hosseinpour Feizi MA, Zarrini G, Tahmasebi M. Evaluation of Protein A Gene Tandem Repeat Polymorphism of Staphylococcus aureus Isolated From Bovine Mastitis in Tabriz. Jundishapur J Microbiol. 2013;6(6):e7934. doi: https://doi.org/10.5812/jjm.7934
1
Jun
2014
Staphylococcus aureus Typing by Digestion of Protein A Coding Gene Using Bsp143I
Fatemeh Shakeri,
Ezzat Allah Ghaemi,
Maya Babai Kochkaksaraei
Shakeri F, Ghaemi EA, Babai Kochkaksaraei M. Staphylococcus aureus Typing by Digestion of Protein A Coding Gene Using Bsp143I. Jundishapur J Microbiol. 2014;7(6):e10320. doi: https://doi.org/10.5812/jjm.10320
Download PDF600.75 KB
Crossmark
Checking
Comments
Number of Comments:0Cited by
- Scopus by DOI: 12
Last Update: 2 weeks ago
- Scopus by Title: 12
Last Update: 2 weeks ago
- Scopus by Title (Ref): 12
Last Update: 2 weeks ago
- CrossRef: 9
Last Update: 4 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Search Relations
Author(s):
Related Articles