Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumoniae

How to Cite:Seyedpour SM, eftekhar F. Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumoniae. Jundishapur J Microbiol. 2014;7(7):e94146. doi: https://doi.org/10.5812/jjm.11136

Abstract

Background: Plasmid-mediated quinolone resistance genes (PMQR) have been shown to play not only an important role in quinolone resistance, but also resistance to other antibiotics, particularly β-lactams and aminoglycosides. These genes are mainly associated with clinical isolates of Enterobacteriaceae. However, detection of PMQR genes in the community isolates can increase the dissemination rate of resistance determinants among bacteria.

Objectives: This study aimed to investigate quinolone resistance and distribution of qnr and aac (6’)-Ib-cr genes among the community isolates of Klebsiella pneumoniae.

Materials and Methods: Fifty-two K. pneumoniae isolates were collected from the Central Laboratory in Karaj between July 2010 and January 2011. Antibacterial susceptibility was determined by the disc diffusion method. Quinolone and/or cephalosporin-resistant isolates were screened for the presence of qnrA, qnrB, qnrS and aac (6’)-Ib-cr genes by polymerase chain reaction (PCR).

Results: Of the 52 K. pneumoniae isolates, 23 were resistant to cephalosporins and/or quinolones. Overall, 7 out of the 23 resistant isolates harbored qnr and/or aac (6’)-Ib-cr genes (30.4%). Among these, 5 isolates were resistant to both classes of antibiotics of which; 3 carried the aac (6’)-Ib-cr gene, one had the qnrS, and one harbored both aac (6’)-Ib-cr and qnrB genes. None of the isolates contained qnrA. Two isolates were sensitive to quinolones and resistant to cephalosporins of which; one had qnrS and the other carried the aac (6’)-Ib-cr gene.

Conclusions: Our study showed that 30.4% of the quinolone and/or cephalosporin resistant community isolates of K. pneumoniaecarried PMQR genes. These results confirm that community isolates can be an important source for spreading antibiotic resistance determinants among Gram negative pathogens. This is the first report from Iran on detection of PMQR in the community isolates of K. pneumoniae.

Keywords

aac (6’)-Ib-cr qnr Genes

Fulltext

Fulltext is available in pdf file.

References

1.
The references are available in PDF file.

Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014

Mohsen Heidary,

Hossein Goudarzi,

Ali Hashemi,

Gita Eslami,

Mehdi Goudarzi,

Alireza Salimi Chirani

,et al.

Heidary M, Goudarzi H, Hashemi A, Eslami G, Goudarzi M, et al. Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014. Arch Pediatr Infect Dis. 2017;5(4):e38343. doi: https://doi.org/10.5812/pedinfect.38343

17

Aug

2022

Prevalence of Plasmid-Mediated Quinolone Resistance Genes and Molecular Typing of Klebsiella pneumoniae Isolates from Blood Cultures in Milad Hospital, Tehran, Iran, Within 2018 - 2019

Maryam Ghane,

Laleh Babaeekhou,

Morteza Asgharifard

Ghane M, Babaeekhou L, Asgharifard M. Prevalence of Plasmid-Mediated Quinolone Resistance Genes and Molecular Typing of Klebsiella pneumoniae Isolates from Blood Cultures in Milad Hospital, Tehran, Iran, Within 2018 - 2019. Jundishapur J Microbiol. 2022;15(7):e124054. doi: https://doi.org/10.5812/jjm-124054

26

Apr

2017

The Impact of Mutations in Topoisomerase Genes and the Plasmid-Mediated Quinolone Resistance (PMQR) Determinants on the Resistance to Fluoroquinolones in Klebsiella pneumoniae

Alisha Akya,

Roya Chegene Lorestani,

Azam Elahi,

Keyghobad Ghadiri

Akya A, Chegene Lorestani R, Elahi A, Ghadiri K. The Impact of Mutations in Topoisomerase Genes and the Plasmid-Mediated Quinolone Resistance (PMQR) Determinants on the Resistance to Fluoroquinolones in Klebsiella pneumoniae. Arch Clin Infect Dis. 2017;12(4):e57290. doi: https://doi.org/10.5812/archcid.57290

16

Mar

2025

Investigating the Frequency of Genotypic and Phenotypic Resistance to Plasmid-Mediated Ciprofloxacin in Clinical Isolates of Klebsiellapneumoniae in Ardabil

Malek Namaki Kheljan,

Maliheh Hasanzadeh,

Zohreh Neyestani,

Mohammad Ali Jafarizare,

Kazem Nejati-Koshki,

Mehdi Asghari Vostakoli

Namaki Kheljan M, Hasanzadeh M, Neyestani Z, Jafarizare MA, Nejati-Koshki K, et al. Investigating the Frequency of Genotypic and Phenotypic Resistance to Plasmid-Mediated Ciprofloxacin in Clinical Isolates of Klebsiellapneumoniae in Ardabil. Arch Clin Infect Dis. 2025;20(3):e148969. doi: https://doi.org/10.5812/archcid-148969

21

Sep

2020

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Siavash Vaziri,

Mandana Afsharian,

Feizollah Mansouri,

Mohsen Azizi,

Fatemeh Nouri,

Nahid Madadi-Goli

,et al.

Vaziri S, Afsharian M, Mansouri F, Azizi M, Nouri F, et al. Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran. Jundishapur J Microbiol. 2020;13(7):e100348. doi: https://doi.org/10.5812/jjm.100348

Import into EndNote Import into BibTex

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 31
Last Update: 5 days ago
Scopus by Title: 26
Last Update: 5 days ago
Scopus by Title (Ref): 31
Last Update: 5 days ago
CrossRef: 19
Last Update: 3 days ago

Metrics

Licensing and reuse information

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Seyed Mohsen Seyedpour:[PubMed][Scholar]
fereshteh eftekhar:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

Outlines

Quinolone Susceptibility and Detection of qnr and aac(6’)-Ib-cr Genes in Community Isolates of Klebsiella pneumoniae

Abstract

Fulltext

References

Similar Articles

Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014

Prevalence of Plasmid-Mediated Quinolone Resistance Genes and Molecular Typing of Klebsiella pneumoniae Isolates from Blood Cultures in Milad Hospital, Tehran, Iran, Within 2018 - 2019

The Impact of Mutations in Topoisomerase Genes and the Plasmid-Mediated Quinolone Resistance (PMQR) Determinants on the Resistance to Fluoroquinolones in Klebsiella pneumoniae

Investigating the Frequency of Genotypic and Phenotypic Resistance to Plasmid-Mediated Ciprofloxacin in Clinical Isolates of Klebsiellapneumoniae in Ardabil

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Crossmark

Checking

Last Update: 5 days ago

Last Update: 5 days ago

Last Update: 5 days ago

Last Update: 3 days ago