Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
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Cytotoxic effects and morphological changes of silver nanoparticles in CHO-K1 cells
Author(s):
Masoumeh Heshmati1, Sepideh Arbabi Bidgoli2,*, Samideh Khoei3, Seyed Mahdi Rezayat4, Kazem Parivar 11Dept. of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Dept. of Toxicology and Pharmacology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
3Medical Physics Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4Dept. of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
*Corresponding Author: Corresponding author: Sepideh Arbabi Bidgoli, Dept. of Toxicology and Pharmacology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran, Tel: +982122640051-55 Email: [email protected]
Published online:Nov 19, 2015
Article type:Research Article
Received:May 04, 2015
Accepted:Oct 06, 2015
How to Cite:Heshmati M, Arbabi Bidgoli S, Khoei S, Rezayat SM, Parivar K. Cytotoxic effects and morphological changes of silver nanoparticles in CHO-K1 cells. J Kermanshah Univ Med Sci. 2015;19(5):e69814. doi: https://doi.org/10.22110/jkums.v19i5.2425
Abstract
Background: Silver nanoparticles are of interest to be used as antimicrobial agents in medical care, wound dressings and cosmetics. Despite the fact that AgNPs are among the most commercialized nano materials owing to their specific antimicrobial properties, there is limited information about their risk assessment and possible hazards to human health and environment. Thus, this study was carried out to evaluate the cytotoxicity and morphological changes of different concentrations of two samples of commercialized silver nanoparticles (A and B) in CHO-K1 cells using MTT assay.
Methods: The cytotoxicity effect of silver nanoparticles AgNP-A (19.6 nm in diameter) and AgNP-B (15 nm in diameter) on CHO-K1 was evaluated in the range of 0.005-500 µg/ml after 24 hours of treatment using MTT assay. The morphological changes of treated cells were examined by light microscopy.
Results: Based on the results of cytotoxicity by MTT assay, reduced viability of cells and cytotoxicity were observed. The 50% inhibitory concentration (IC50) of silver nanoparticles was recorded at 100 and 10 µg/ml for AgNP-A and AgNP-B, respectively. Moreover, microscopic observations indicated clear morphological changes of treated cells.
Conclusion: Concentration and physicochemical properties of silver nanoparticles like size, shape, surface area, coating and zeta potential play a role in cytotoxicity and morphological changes of treated cells.
References
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Copyright
© 2015, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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