Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
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Studies of some cancer markers and blood factors in DMBA- induced breast cancer in SD rat strains
Author(s):
1Dept. of Biology, School of Sciences, Arak University, Arak, Iran
*Corresponding Author: Corresponding author: Ahmad Hamta, Dept. of Biology, School of Sciences, Arak University, Arak, Iran, Tel: +988614173414 Email: [email protected]
Published online:Sep 19, 2011
Article type:Research Article
Received:Jun 08, 2010
Accepted:Nov 02, 2010
How to Cite:Hamta A, Shariatzadeh SMA, Solimani Mehrnejani M. Studies of some cancer markers and blood factors in DMBA- induced breast cancer in SD rat strains. J Kermanshah Univ Med Sci. 2011;15(3):e79360. doi:
Abstract
Background: Animal suitable models are very useful tools in the studies of complicated diseases. In this way we can study the heterogeneity of human genome and also we can minimize the effect of environmental factors in the incidence of cancers. In this study the expression of VWF and PCNA in tumors was determined by immunohistochemistry and also we measured three cancer markers and two blood factors from affected rats.
Methods: We have gavaged 10 mg DMBA dissolved in 0.5 ml sesame oil to the DMBA-treated SD rat strains. After tumor were appeared tumors were used for immunohistochemistry tests. Also we measured three cancer markers and two blood factors according to the manufacturer's protocol and results were analyzed by SPSS software.
Results: Interaction of two antibodies PCNA and VWF with their antigen were more robust in tumors samples compared to controls with control samples. From blood factors only in tumors CA13.5 showed significant expression (p<0.05).
Conclusion: Histopathology and immunohistochemistry results showed that breast cancer by induced DMBA in rats were similar to human breast cancer. The level of CA15.3 in induced tumors showed significantly increase compared to control blood samples, which is similar to behavior of this cancer marker in human breast cancer. But there was no significant association between treated and control samples related to the other four cancer markers and blood factors These results emphasized that DMBA-induced breast cancer in SD rat strains is a suitable model for biological studies on breast cancers.
References
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Copyright
© 2011, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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