Determination of Levonorgestrel and Ethinyl Estradiol in Pharmaceutical Formulations by H-Point Standard Addition Method in Nonaqueous Solvent Using Simultaneous Addition of Both Analytes
Authors
Abstract
H-point standard addition method (HPSAM) with simultaneous addition of both analytes in nonaqueous solution was applied for determination of levonorgestrel (LEV) and ethinyl estradiol (EE) in LD and HD tablets. The results showed that, simultaneous determination could be performed with the ratio 1 to 5 of LEV-EE. The LOD for LEV and EE were obtained 5.1×10-7 and 9.6×10-7 M, respectively, and the corresponding values of %RSD (n=4) for LEV and EE were 1.96 and 2.69, respectively. Underworking conditions, the proposed method was successfully applied for simultaneous determination of LEV and EE in several synthetic mixtures and LD and HD tablets.
Highlights
Copyright
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
HPTLC-Densitometric Determination of Cetirizine and Montelukast Analysis in Combined Tablet Dosage Forms
Haghighi S, Shapouri MR, Amoli-Diva M, Pourghazi K, Afruzi H. HPTLC-Densitometric Determination of Cetirizine and Montelukast Analysis in Combined Tablet Dosage Forms. Iran J Pharm Res. 2013;12(2):e125708. doi: https://doi.org/10.22037/ijpr.2013.1292
Quantitative Determination of Levofloxacin in Ophthalmic Solution by High-Performance Liquid Chromatography
Salih Mohammed S, Abazari M, Fattah Aziz D, Hama Salh HJ, Ali Salih M, et al. Quantitative Determination of Levofloxacin in Ophthalmic Solution by High-Performance Liquid Chromatography. Jundishapur J Nat Pharm Prod. 2025;20(4):e160726. doi: https://doi.org/10.5812/jjnpp-160726
A Simple Sample Preparation with HPLC-UV Method for Estimation of Clomipramine from Plasma
Mostafavi SA, Tahvilian R, Dehghani Poudeh M, Rafeepour Z. A Simple Sample Preparation with HPLC-UV Method for Estimation of Clomipramine from Plasma. Iran J Pharm Res. 2010;9(3):e126045. doi: https://doi.org/10.22037/ijpr.2010.862
Quantitative Bioanalytical and Analytical Methods for Estimation of Ivabradine Hydrochloride in Pure and Pharmaceutical Dosage Form
Polker A, Padmavathi Y, Kumar R, Raghavendra Babu N. Quantitative Bioanalytical and Analytical Methods for Estimation of Ivabradine Hydrochloride in Pure and Pharmaceutical Dosage Form. J Rep Pharm Sci. 2020;9(1):e147274. doi: https://doi.org/10.4103/jrptps.JRPTPS_110_19
Simultaneous Analysis and Efficient Separation of Anabolic Androgenic Steroids in Dietary Supplement by a Validated HPTLC Method
Saadabadi Z, Daraei B, Kobarfard F, Amirahmadi M, Kheirollahi K. Simultaneous Analysis and Efficient Separation of Anabolic Androgenic Steroids in Dietary Supplement by a Validated HPTLC Method. Iran J Pharm Res. 2022;21(1):e127444. doi: https://doi.org/10.5812/ijpr-127444
- Scopus by DOI: 0
Last Update: 2 weeks ago
- Scopus by Title: 3
Last Update: 2 weeks ago
- Scopus by Title (Ref): 3
Last Update: 2 weeks ago
- CrossRef: 0
Last Update: 7 hours ago
- OpenAlex: 0
Last Update: 1 day ago
- OpenCitations: 0
Last Update: 5 days ago