Journal of Semnan University of Medical Sciences
Image Credit:Koomesh
Outline
Investigating the Role of Tumor-Associated Macrophages in the Resistance of Brain Metastatic Tumor Cells to Paclitaxel in Two- Dimensional and Three-Dimensional Cell Culture Conditions
Authors
Abstract
Background: Brain metastasis is a devastating complication in breast cancer patients. Tumor-associated macrophages (TAMs) are cells in innate immunity, repair, and homeostasis. These cells play an important role in drug resistance in the metastatic microenvironment.
Objectives: In this study, the roles of TAMs in the resistance of brain metastatic cells to paclitaxel were evaluated in two-dimensional and three-dimensional cell culture conditions
Methods: After the generation of a mouse model of breast cancer, brain metastatic tumor cells named 4T1B were isolated from brain metastasis lesions in mice. Simultaneous cultivation of RAW macrophage cells and 4T1B tumor cells was done in 2D and 3D (multicellular spheroids) culture mediums. The cytotoxic activity of paclitaxel on cells was evaluated by MTT assay.
Results: The results of this study show that TAMs cause a significant increase in paclitaxel resistance in brain metastatic cells only in 3D cell culture. Also, spheroidal formation was greater in terms of number and size when metastatic cells and macrophages were cultured simultaneously.
Conclusion: Therefore, according to the results of this study, it can be concluded that tumor-related macrophages increased brain metastatic cells in terms of spheroid formation, which indicates that they are cultured in three-dimensional conditions. Also, these cells reduce the response to treatment, or, in other words, drug resistance. These results may provide useful insights for future studies in cancer chemoresistance.
Highlights
References
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© 2025, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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