Koomesh
Journal of Semnan University of Medical Sciences
Proteomics Study of extremely low frequency electromagnetic field (50 Hz) on human neuroblastoma cells
Abstract
Introduction: Extremely low frequency electromagnetic field (ELF-EMF) is one of common environmental factors in today’s modern life. In recent years, a special interest has been directed toward the possible effects of ELF-EMFs on biological systems. Since the mechanism underlying the ELF-EMFs’ biological effects are still unknown, we decided to conduct an in vitro study to understand the possible mechanisms. Material and Methods: SH-SY5Y- human neuroblastoma cell line were placed in the EL-EM Field with the intensity of 1 mT and frequency of 50 Hz and for a period of 4 hours/day, in 2 days period. The cell morphology and protein expression were measured by using proteomic methods. Statistical analysis was performed by using Non Linear Spot Progenesis Same software. Results : Our results showed that ELF-EMF could likely change the cell morphology and proliferation. Conclusion: Statistical analysis in our study showed that ELF-EMF with a frequency of 50Hz and intensity of 1mT could significantly change the protein expression (P ;le 0.05).
Copyright
© 2015, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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