Journal of Semnan University of Medical Sciences
Image Credit:Koomesh
Outline
Comparative investigation of the culture of palatine tonsils by superficial and deep methods and investigation of microbial resistance in frequent tonsillitis patients referred for tonsillectomy
Authors
, Sara Khashkhashi Moghaddam, Kiarash Ghazvini, Negar Morovatdar, Mohamad Afzalzadeh, Bashir Rasoulian,*Abstract
Introduction: The tonsils are part of the immune system and tonsillar infection is one of the most common causes of diseases, especially in children. Tonsillitis sometimes does not respond to empirical treatment and the presentation of recurrent tonsillitis eventually leads to tonsillectomy. The aim of this study was to determine and compare palatal tonsil culture by superficial and deep methods in frequent tonsillitis patients referred for tonsillectomy. Materials and Methods: This cross-sectional study was performed on all patients aged > 3 years with chronic recurrent tonsillitis referred for tonsillectomy during 2019-2020. After obtaining the informed consent of the patient's legal guardian, the patient underwent sampling of the right palatine tonsil with a swab through the mouth before tonsillectomy. After tonsillectomy, a sampling of the surface and deep tissue of the tonsil was performed. Microbiological examination, antibiogram determination, and blood culture before and after tonsillectomy were performed. Results: A total of 80 patients were recruited in this study of which 50% were male. In 87.1% of patients, the report of staphylococcus (as the most common organism found) had poor consistency in both surface and deep culture methods. This rate reached 81.3% in comparison between deep tissue culture results and secretions (swab) and 72.7% in comparison between surface samples and secretions (swab) (P<0.001). There was the lowest amount of negative cultures in the comparison of 3 surface cultures, deep cultures and secretions (swap) in deep samples. The highest antibiotic susceptibilities against tonsillar organisms were observed in clindamycin, erythromycin, cefoxitin, gentamicin, and doxycycline. Conclusion: Blood cultures before and after tonsillectomy were negative in all samples. Based on the results, it seems that no persistent infection enters the blood during tonsillectomy in otherwise healthy patients. It also seems that the use of deep culture is more beneficial compared to surface culture because it has a lower false negative rate.
Highlights
References
- 1.Dostmohammadian N, Danaee N, Ghorbani R, Rahimi N. Helicobacter pylori colonization in children with Adenotansillar hypertrophy. Koomesh 2018; 20: 688-691. (Persian).
- 2.Sarkar S, Sil A, Sarkar S, Sikder B. A comparison of tonsillar surface swabbing, fine-needle aspiration core sampling, and dissected tonsillar core biopsy culture in children with recurrent tonsillitis. Ear Nose Throat J 2017; 96: E29-e32.
- 3.Baugh RF, Archer SM, Mitchell RB, Rosenfeld RM, Amin R, Burns JJ, et al. Clinical practice guideline: tonsillectomy in children. Otolaryngol Head Neck Surg 2011; 144: S1-S30.
- 4.umilo D, Nichols L, Ryan R, Marshall T. Incidence of indications for tonsillectomy and frequency of evidence-based surgery. Br J Gen Pract 2019; 69: e33-e41.
- 5.Abdollahi Fakhim S, Dostmohammadian N, Davarmoin G. Survey of postoperative hemorrhage after tonsillectomy and adenoidectomy in children. Koomesh 2007; 8: 111-116. (Persian).
- 6.Gul M, Okur E, Ciragil P, Yildirim I, Aral M, Kilic MA. The comparison of tonsillar surface and core cultures in recurrent tonsillitis. Am J Otolaryngol 2007; 28: 173-176.
- 7.Rekabi H, Khosravi A, Ahmadi K, Kardouni M. The microbiologic comparison of the surface and deep tissue tonsillar cultures in patients underwent tonsillectomy. J Med Sci 2008; 8: 325-328.##https://doi.org/10.3923/jms.2008.325.328.
- 8.Jayasimha VL, Vinodkumar CS, Raghukumar KG, Basavarajappa KG. Surface Tonsillar bacteria versus deep tonsillar bacteria in tonsillitis. J Pub Health Med Res 2013; 1: 92-94.
- 9.Yildirim I, Okur E, Ciragil P, Aral M, Kilic MA, Gul M. Bacteraemia during tonsillectomy. J Laryngol Otol 2003; 117: 619-623.
- 10.Manandhar S, Bhandary S, chettri St, Khanal B, Shah S, Sah BP, Paudel D. Bacteriological evaluation of tonsillar surface and tonsillar core micro flora in patients undergoing tonsillectomy. Health Renaiss 2016; 12: 149.##https://doi.org/10.3126/hren.v12i3.15252.
- 11.Babaiwa UF, Onyeagwara NC, Akerele JO. Bacterial tonsillar microbiota and antibiogram in recurrent tonsillitis. Biomed Res 2013; 24: 298-302.
- 12.Klug TE, Henriksen JJ, Rusan M, Fuursted K, Ovesen T. Bacteremia during quinsy and elective tonsillectomy: an evaluation of antibiotic prophylaxis recommendations for patients undergoing tonsillectomy. J Cardiovasc Pharmacol Ther 2012; 17: 298-302.
- 13.Esposito S, Marchisio P, Capaccio P, Bellasio M, Semino M, Dusi E, et al. Risk factors for bacteremia during and after adenoidectomy and/or adenotonsillectomy. J Infect 2009; 58: 113-118.
- 14.Kaygusuz Ir, Gk , Yalin , Kele E, Kizirgil A, Demirba E. Bacteremia during tonsillectomy. Int J Pediatr Otorhinolaryngol 2001; 58: 69-73.
- 15.Shishegar M, Ashraf MJ. Posttonsillectomy bacteremia and comparison of tonsillar surface and deep culture. Adv Prev Med 2014; 2014.
Copyright
© 2023, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Frequency of Helicobacter pylori in paraffin embedded blocks of chronic tonsillitis patients (2008-2009)
Pahlevan A, Ahmadpour-Qazvini F, Naserpour Farivar T, Johari P, Safdarian F, et al. Frequency of Helicobacter pylori in paraffin embedded blocks of chronic tonsillitis patients (2008-2009). J Inflamm Dis. 2024;16(3):e155705. doi:
Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications
Pourmousa R, Varshoi F, Nikkhah M, Ebrahimzadeh P, Rezai S, et al. Helicobacter pylori Colonization in Patients with Adenotonsillar Hypertrophy: Study on Prevalence and Clinical Characteristics of Colonized Patients and Possible Association with Complications. Arch Pediatr Infect Dis. 2020;8(2):e97561. doi: https://doi.org/10.5812/pedinfect.97561
The study of serum and tissue cholesterol levels in children undergoing tonsillectomy
Shaaker M, Mehdizadeh A, Ezzeddini R, Ghasemi B, Jabbari Moghaddam Y, et al. The study of serum and tissue cholesterol levels in children undergoing tonsillectomy. J Inflamm Dis. 2024;22(4):e156098. doi:
Incidence, Risk Factors, and Management Approaches for Post-tonsillectomy Hemorrhage: A Systematic Review
Farshchian N, Karaji S. Incidence, Risk Factors, and Management Approaches for Post-tonsillectomy Hemorrhage: A Systematic Review. J Kermanshah Univ Med Sci. 2025;29(3):e163907. doi: https://doi.org/10.5812/jkums-163907
Identification and Detection of Pathogenic Bacteria in Adenoid Tissue of Adenoidectomized Children: Emergence of Staphylococcus aureus as the Most Prevalent Pathogen
Sabz G, Moradi S, Sharifi A, Naghmachi M, Taheripour Sisakht M, et al. Identification and Detection of Pathogenic Bacteria in Adenoid Tissue of Adenoidectomized Children: Emergence of Staphylococcus aureus as the Most Prevalent Pathogen. Jundishapur J Microbiol. 2020;13(1):e95445. doi: https://doi.org/10.5812/jjm.95445
- Scopus by DOI: 0
Last Update: 3 weeks ago
- Scopus by Title: 0
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 6 hours ago
- OpenAlex: 0
Last Update: 8 hours ago
- OpenCitations: 0
Last Update: 4 days ago
