Journal of Semnan University of Medical Sciences
Image Credit:Koomesh
Outline
Activity of biofilm genes icaA and icaR in methicillin-resistant Staphylococcus aureus treated with vitamin K in wound specimens
Authors
Abstract
Introduction: The use of vitamin K plays a very important role in human health. This vitamin may have effects on some of the pathogens such as biofilms in Staphylococcus aureus.In this account, the purpose of this study was to determine the effect of vitamin K on the expression of icaA and icaR genes in methicillin-resistant S. aureus isolated from the wound sample. Materials and Methods: In this interventional study, 15 isolates of methicillin-resistant S. aureus were treated with vitamin K at concentrations of 1, 10, 50, 100, 200, 300 and 500 μg / ml. Time intervals for incubation were determined 24, 48 and 72 hours. Finally, the desired RNA was extracted and cDNA was synthesized. The expressions of icaA and icaR genes were evaluated using qPCR method. Results: The expression levels of icaA and icaR genes were used as controls prior to vitamin treatment. Both of genes showed a reduced expression rate in isolates which treated by vitamin K. This effect on the expression of icaA and icaR was clearly observed at a concentration of 200 μg and also in the 48-hour incubation. Conclusion: Vitamin K along with some antibiotics could be effective against some certain skin infections which caused by methicillin resistance S. aureus.
Highlights
References
- 1.Zarei Koosha R, Mahmoodzadeh Hosseini H, Mehdizadeh Aghdam E, Ghorbani Tajandareh S, Imani Fooladi AA. Distribution of tsst-1 and mecA Genes in Staphylococcus aureus Isolated From Clinical Specimens. Jundishapur J Microbiol 2016; 9: e29057. (Persian).
- 2.Abdal N, Ghaznavirad E, Hamidi A, Hosseini D. Prevalence of genes encoding aminoglycoside resistant in methicillin-sensitive Staphylococcus aurous and coagulase-negative staphylococci isolated from hospital infectious. Koomesh 2014; 16: 82-89. (Persian).
- 3.Heydari N, Alikhani MY, Azizi Jalilian F, Tahmasebi H, Arabestani MR. Evaluation of real time PCR for detection of clinical isolates of Staphylococcus aureus and methicillin-resistance strains based on melting curve analysis method. Koomesh 2017; 19: 877-886. (Persian).
- 4.Tahmasebi H, Zeiyni B, Dehbashi S, Motamedi H, Vafaeifar M, Keramat F, Arabestani MR. The study of blaZ and mecA gene expression in methicillin-resistant staphylococcus aureus strains and the relationship between the gene expression Patterns. 2017; 2017: 6. (Persian).
- 5.Gschwind L, Rollason V, Daali Y, Bonnabry P, Dayer P, Desmeules JA. Role of P-glycoprotein in the uptake/efflux transport of oral vitamin K antagonists and rivaroxaban through the Caco-2 cell model. Basic Clin Pharmacol Toxicol 2013; 113: 259-265.
- 6.Hammond RK, White DC. Inhibition of vitamin K2 and carotenoid synthesis in Staphylococcus aureus by diphenylamine. J Bacteriol 1970; 103: 611-615.
- 7.Gewin HM, Friou GJ. Manifestations of vitamin deficiency during aureomycin and chloramphenicol therapy of endocarditis due to Staphylococcus aureus; report of a case. Yale J Biol Med 1951; 23: 332-338.
- 8.Chen X, Shang F, Meng Y, Li L, Cui Y, Zhang M, Qi K, Xue T. Ethanol extract of Sanguisorba officinalis L. inhibits biofilm formation of methicillin-resistant Staphylococcus aureus in an ica-dependent manner. J Dairy Sci 2015; 98: 8486-8491.
- 9.Zhu Y, Weiss EC, Otto M, Fey PD, Smeltzer MS, Somerville GA. Staphylococcus aureus biofilm metabolism and the influence of arginine on polysaccharide intercellular adhesin synthesis, biofilm formation, and pathogenesis. Infect Immun 2007; 75: 4219-4226.
- 10.Ferreira FA, Souza RR, Bonelli RR, Americo MA, Fracalanzza SE, Figueiredo AM. Comparison of in vitro and in vivo systems to study ica-independent Staphylococcus aureus biofilms. J Microbiol Methods 2012; 88: 393-398.
- 11.Szweda P, Schielmann M, Milewski S, Frankowska A, Jakubczak A. Biofilm production and presence of ica and bap genes in Staphylococcus aureus strains isolated from cows with mastitis in the eastern Poland. Pol J Microbiol 2012; 61: 65-69.
- 12.Kirby DT, Savage JM, Plotkin BJ. Menaquinone (Vitamin K2) Enhancement of Staphylococcus aureus Biofilm Formation. J Bios Med 2014; 12: 26-32.
- 13.Andrade JC, Morais Braga MF, Guedes GM, Tintino SR, Freitas MA, Quintans LJ, et al. Menadione (vitamin K) enhances the antibiotic activity of drugs by cell membrane permeabilization mechanism. Saudi J Biol Sci 2017; 24: 59-64.
- 14.DW sJaR. Molecular cloninig:a laboratory manual. 3 ed: cold spring harbor laboratory press; 2001.
- 15.Ares M. Bacterial RNA isolation. Cold Spring Harb Protoc 2012; 2012: 1024-1027.
- 16.Pfaffl WM. A new mathematical model for relative quantification in real-time RTPCR. Nucleic Acids Res 2001; 29: e45.
- 17.Schlievert PM, Merriman JA, Salgado-Pabn W, Mueller EA, Spaulding AR, Vu BG, et al. Menaquinone Analogs Inhibit Growth of Bacterial Pathogens. Antimicrob Agents Chemother 2013; 57: 5432-5437.
- 18.Mirzaie A, Noorbazargan H, Khatami SHRK, Sadat Shandiz SA, Rahimi A, Bagheri keshtali Aa. Evaluation of chemical composition of helichrysum artemisioides extract its effect on biofilm formation and IcaD gene expression in clinical Isolates of methicillin-resistant staphylococcus aureus. J Ilam Univ Med Sci 2017; 25: 180-194. (Persian).
- 19.Lee KM, Go J, Yoon MY, Park Y, Kim SC, Yong DE, Yoon SS. Vitamin B12-mediated restoration of defective anaerobic growth leads to reduced biofilm formation in Pseudomonas aeruginosa. Infect Immun 2012; 80: 1639-1649.
- 20.Yarwood JM, Bartels DJ, Volper EM, Greenberg EP. Quorum sensing in Staphylococcus aureus biofilms. J Bacteriol 2004; 186: 1838-1850.
- 21.Osman KM, Amer AM, Badr JM, Helmy NM, Elhelw RA, Orabi A, et al. Antimicrobial resistance, biofilm formation and mecA characterization of methicillin-susceptible S. aureus and Non-S. aureus of beef meat origin in egypt. Front Microbiol 2016; 7: 222.##.
Copyright
© 2018, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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