Cover image of the article: Activity of biofilm genes icaA and icaR in methicillin-resistant Staphylococcus aureus treated with vitamin K in wound specimens

Image Credit:Koomesh

Activity of biofilm genes icaA and icaR in methicillin-resistant Staphylococcus aureus treated with vitamin K in wound specimens

Authors

Naime Kashefi pasandideh, Mohammad Reza Habibi, Hamed Tahmasebi, mohammad reza arabestani,*
*Corresponding Author: Email: mohammad.arabestani@gmail.com

Koomesh:Vol. 20, issue 3; 588-593
Published online:Aug 06, 2018
Article type:Research Article
Received:Sep 01, 2017
Accepted:Feb 06, 2018
How to Cite:Kashefi pasandideh N, Habibi MR, Tahmasebi H, arabestani MR. Activity of biofilm genes icaA and icaR in methicillin-resistant Staphylococcus aureus treated with vitamin K in wound specimens. koomesh. 2018;20(3):e152998. doi:

Abstract

Introduction: The use of vitamin K plays a very important role in human health. This vitamin may have effects on some of the pathogens such as biofilms in Staphylococcus aureus.In this account, the purpose of this study was to determine the effect of vitamin K on the expression of icaA and icaR genes in methicillin-resistant S. aureus isolated from the wound sample. Materials and Methods: In this interventional study, 15 isolates of methicillin-resistant S. aureus were treated with vitamin K at concentrations of 1, 10, 50, 100, 200, 300 and 500 μg / ml. Time intervals for incubation were determined  24, 48 and 72 hours. Finally, the desired RNA was extracted and cDNA was synthesized. The expressions of icaA and icaR genes were evaluated using qPCR method. Results: The expression levels of icaA and icaR genes were used as controls prior to vitamin treatment. Both of genes showed a reduced expression rate in isolates which treated by vitamin K. This effect on the expression of icaA and icaR was clearly observed at a concentration of 200 μg and also in the 48-hour incubation. Conclusion: Vitamin K along with some antibiotics could be effective against some certain skin infections which caused by methicillin resistance S. aureus.

Highlights

References

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Copyright

© 2018, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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