Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran

Alireza Ekrami; Alireza Samarbafzadeh; Mohammad Alavi; Enayat Kalantar; Farhad Hamzeloi

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

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Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran

Author(s):

Alireza Ekrami^1,*,

Alireza Samarbafzadeh²,

Mohammad Alavi²,

Enayat Kalantar³,

Farhad Hamzeloi²

1Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences, [email protected], Iran

2Infectious and Tropical Diseases Research Center, Jundishapur University of Medical Sciences, Iran

3Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Iran

Jundishapur Journal of Microbiology:Vol. 3, issue 2; 84-91

Published online:Apr 30, 2010

Article type:Research Article

Received:Jan 01, 2010

Accepted:Apr 01, 2010

How to Cite:Ekrami A, Samarbafzadeh A, Alavi M, Kalantar E, Hamzeloi F. Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran. Jundishapur J Microbiol. 2010;3(2):. doi:

Abstract

Introduction and objective: Today methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase negative Staphylococci (MRCNS) are frequent causes of nosocomial infection. Extensive burn injuries, extended hospitalization and inappropriate antibiotic therapy have been identified as risk factors for MRSA and MRCNS carriage and infection. The aim of this study was to assess the prevalence of methicillin resistance among clinical isolates of Staphylococci taken from burn patients using four separated methods and also determination of susceptibility pattern to amikacin, ciprofloxacin, vancomycin, carbenicillin and gentamicin.

Materials and methods: A total of 185 clinical staphylococcal isolates from wound and blood specimens were evaluated for susceptibility to oxacillin using oxacillin and cefoxitin disk diffusion method, agar screening containing 6 microgram oxacillin /ml, oxacillin E test and polymerase chain reaction for predicting mecA gene.

Results: The results showed that 27.8% of wound and blood specimens were infected by Staphylococci and among these 60% were identified as methicillin resistant. We found no significant differences between the results of PCR assay and conventional disk diffusion method by oxacillin and cefoxitin disk, however the results of cefoxitin disk was more significant than oxacillin and gave better results. Both of the sensitivity and specificity value were similar (99%, 100%) for E test and agar screen test. Furthermore in E test for detection of minimum inhibitory concentration (MIC), more than 93% of MRSA and 15% of MRCNS isolates had MIC value more than 256µg/ml. We also determined a significant difference pattern between methicillin resistant and methicillin susceptible Staphylococci to five antimicrobial agents.

Conclusion: In conclusion, our results showed that the prevalence of methicillin resistant Staphylococci in our center was very high and cefoxitin disk test is reliable alternation for detection of methicillin resistant Staphylococci.

Keywords

Staphylococcus aureus

Coagulase-negative Staphylococci

mecA

Cefoxitin

Oxacillin

Methicillin resistance

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Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients

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Abbas Akhavan Sepahi,

Hossein Goudarzi,

Mahboobeh Satarzadeh Tabrizi,

Mehdi Goudarzi,

Bahareh Hajikhani

,et al.

Ghaderi Afshari S, Akhavan Sepahi A, Goudarzi H, Satarzadeh Tabrizi M, Goudarzi M, et al. Distribution of SCCmec Types in Methicillin-Resistant Staphylococcus aureus Isolated from Burn Patients. Arch Clin Infect Dis. 2017;12(2):e62760. doi: https://doi.org/10.5812/archcid.62760

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Methicillin-Resistant Staphylococcus aureus Isolated from Central Region of Iran: Antibiotic Resistance and SCCmecA Types

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Fatehi Fazli SA, Fatahi-Bafghii M, Vakili M, Sadeh M. Methicillin-Resistant Staphylococcus aureus Isolated from Central Region of Iran: Antibiotic Resistance and SCCmecA Types. Jundishapur J Microbiol. 2024;17(12):e156747. doi: https://doi.org/10.5812/jjm-156747

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Detection of Methicillin-Resistant Coagulase-Negative Staphylococci and Surveillance of Antibacterial Resistance in a Multi-Center Study from Iran

Mohammad Reza Nahaei,

Mohammad Reza Shahmohammadi,

Shiva Ebrahimi,

Morteza Milani

Nahaei MR, Shahmohammadi MR, Ebrahimi S, Milani M. Detection of Methicillin-Resistant Coagulase-Negative Staphylococci and Surveillance of Antibacterial Resistance in a Multi-Center Study from Iran. Jundishapur J Microbiol. 2015;8(8):e19945. doi: https://doi.org/10.5812/jjm.19945v2

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Nasrollah Sohrabi,

Ramin Abiri,

Sajjad Babaei,

Zohreh Amiri

Hesari Y, Sohrabi N, Abiri R, Babaei S, Amiri Z. Staphylococcal Cassette Chromosome mec Typing of Meticillin-Resistant Staphylococcus aureus in Kermanshah Province, West of Iran. Jundishapur J Microbiol. 2020;13(3):e98852. doi: https://doi.org/10.5812/jjm.98852

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Hossein Goudarzi,

Bahareh Hajikhani

Mohammadi A, Goudarzi M, Dadashi M, Soltani M, Goudarzi H, et al. Molecular Detection of Genes Involved in Biofilm Formation in Staphylococcus aureus Strains Isolates: Evidence From Shahid Motahari Hospital in Tehran. Jundishapur J Microbiol. 2020;13(7):e102058. doi: https://doi.org/10.5812/jjm.102058

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Author(s):

Alireza Ekrami:[PubMed][Scholar]
Alireza Samarbafzadeh:[PubMed][Scholar]
Mohammad Alavi:[PubMed][Scholar]
Enayat Kalantar:[PubMed][Scholar]
Farhad Hamzeloi:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

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Prevalence of methicillin resistant Staphylococcus species isolated from burn patients in a burn center, Ahvaz, Iran

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