Zahedan Journal of Research in Medical Sciences
The Official Journal of Zahedan University of Medical Sciences
Authors
In acute myeloid leukemia (AML), a large number of tumor suppressor genes are silenced through DNA methylation such as CDKN2B and p73. Wnt inhibitory factor 1 (WIF1) and Dickkopf-1 (DKK-1) are negative regulator of the Wnt signaling pathway.
In the present study, we studied the methylation status of WIF1 and DKK-1 genes in AML patients.
In this case-control study, blood samples from 120 AML patients and 25 healthy control subjects collected, isolated DNA was treated with sodium bisulphite and examined by methylation specific PCR (MS-PCR) with primers specific for methylated and unmethylated sequences of the WIF1 and DKK-1 genes.
The frequency of aberrant hypermethylation of WIF1 and DKK-1 genes in AML patients determined 35% (42/120) and 28.3% (34/120), respectively. In addition, for all subjects in control group, methylation of WIF1 and DKK-1 genes were negative. Patients with M0 subtype of French-American-British (FAB)-AML had the highest incidence of hypermethylation of WIF1 (P = 0.003) and DKK-1 (P = 0.005) genes.
The present study showed that, like many solid tumors, WIF1 and DKK-1 genes methylation also occurs in AML. The study of other antagonists of Wnt signaling pathway are recommended.
Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Bakhshipour A. Retraction Note: Methylation of the Wnt Signaling Antagonist, Wnt Inhibitory Factor 1 and Dickkopf-1 Genes in Acute Myeloid Leukemia at the Time of Diagnosis. Zahedan J Res Med Sci. 2023;25(4):e139846. doi: https://doi.org/10.5812/zjrms-139846
Kamali Dolatabadi E, Ostadali Dehaghi M, Amirizadeh N, Parivar K, Mahdian R. CDKN2B Methylation Correlates with Survival in AML Patients. Iran J Pharm Res. 2017;16(4):e125057. doi: https://doi.org/10.22037/ijpr.2017.2111
Rahnemoon AR, Koochakzadeh L, Ansari S, Boyajyan A, Arakelyan A. RETRACTED ARTICLE: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children. Inn J Pediatr. 2018;28(3):e5371. doi: https://doi.org/10.5812/ijp.5371
Safari I, Nourian M, Naghoosi H, Etemadi A, Zeinali F, et al. Analysis of the Bach2 and HDAC3 Expression in Iranian Patients with Acute Myeloid Leukemia. Int J Cancer Manag. 2019;12(12):e91545. doi: https://doi.org/10.5812/ijcm.91545
Walizadeh G. Retraction Note: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children. Inn J Pediatr. 2018;28(6):e85013. doi: https://doi.org/10.5812/ijp.85013
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