Objectives:
The following observation was primarily based on the study of gene fusion in blood and bone marrow cells taken from 68 Iranian children with acute lymphoblastic leukemia (ALL), to compare with healthy population.
Innovative Journal of Pediatrics
The Scientific Journal of Growth & Development Research Center
RETRACTED ARTICLE: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children
Author(s):
1Allied Medical School, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IR Iran
2Children’s Medical Center, Pediatrics Center of Excellence, Tehran, IR Iran
3Department of Pediatrics, Tehran University of Medical Sciences, Tehran, IR Iran
4Department of Pediatrics, Iran University of Medical Sciences, Tehran, IR Iran
5Institute of Molecular Biology NAS RA, Yerevan, Armenia
Published online:May 29, 2018
Article type:Research Article
Received:Jan 12, 2016
Accepted:Jan 27, 2018
This article has been retracted. See Retraction Note: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children.
How to Cite:Rahnemoon AR, Koochakzadeh L, Ansari S, Boyajyan A, Arakelyan A. RETRACTED ARTICLE: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children. Inn J Pediatr. 2018;28(3):e5371. doi: https://doi.org/10.5812/ijp.5371
Abstract
Methods:
Peripheral blood and bone marrow samples obtained from patients with ALL were immunophenotyped to determine the lineage and the level of differentiation. With reverse transcriptase-polymerase chain reaction (RT-PCR), the RNA molecules were analyzed according to Van Dongen et al. protocol to detect fused genes in cell population.
Results:
Leukemic cell type was identified by cytochemical stains and classified on the basis of FAB classification. Nonetheless the frequencies of E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR) and BCR/ABL(m-BCR) gene transcripts were 1.5%, 0%, 0% and 4.4% respectively. The positive case of E2A/PBX1 fusion gene had an early pre B and 3 BCR/ABL (m-BCR). Positive cases had an early pre B and pre-B ALL immunophenotype.
Conclusions:
Early pre-B cells were the most common types in our patients. The RT-PCR was shown to be an ideal method for detecting hybrid transcripts and to estimate the prevalence of the fusion genes in ALL patients. The frequency of these fusion genes in Iranian pediatric ALL patients were found to be similar to some developed countries. Thus, their presence does not seem to be predictive of increasing malignancy, but rather it can challenge the prognostic significance of these rearrangements.
Copyright
Copyright © 2018, Copyright Holder Demo. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
Similar Articles
6
Oct
2018
Retraction Note: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children
Gholam-Reza Walizadeh
Walizadeh G. Retraction Note: E2A/PBX1, MLL/AF4, BCR/ABL (M-BCR), BCR/ABL(m-BCR) Gene Rearrangements in Acute Lymphoblastic Leukemia in Iranian Children. Inn J Pediatr. 2018;28(6):e85013. doi: https://doi.org/10.5812/ijp.85013
31
Jul
2017
The Frequency of BCR-ABL1 Fusion Transcripts in Iranian Patients with Three Different Types of Leukemia
Mohammad Hamid,
Hanieh Bokharaei
Hamid M, Bokharaei H. The Frequency of BCR-ABL1 Fusion Transcripts in Iranian Patients with Three Different Types of Leukemia. Zahedan J Res Med Sci. 2017;19(7):e10197. doi: https://doi.org/10.5812/zjrms.10197
10
Dec
2013
Prevalence of ETV6/RUNX1 Fusion Gene in Pediatric Patients with Acute Lymphoblastic Leukemia in Iran
Ahmad-Reza Rahnemoon,
Farhad Zaker,
Mina Izadyar,
Shahla Ansari,
Behzad Poopak,
Yuri Tadavosyan
Rahnemoon A, Zaker F, Izadyar M, Ansari S, Poopak B, et al. Prevalence of ETV6/RUNX1 Fusion Gene in Pediatric Patients with Acute Lymphoblastic Leukemia in Iran. Inn J Pediatr. 2013;23(6):. doi:
1
Oct
2009
Investigation of TEL/AML1 and BCR/ABL genes fusion in Acute lymphoblastic leukemia (ALL) Patients and Follow-up Study in 25 Bone Marrow Transplanted (BMT) Patients Using Interphase Fluorescence In Situ Hybridization (FISH).
SA Rahmani,
P Mehdipour,
F Aboualsoltani,
M Izadyar,
M Zamani,
AM Aghazadeh
Rahmani S, Mehdipour P, Aboualsoltani F, Izadyar M, Zamani M, et al. Investigation of TEL/AML1 and BCR/ABL genes fusion in Acute lymphoblastic leukemia (ALL) Patients and Follow-up Study in 25 Bone Marrow Transplanted (BMT) Patients Using Interphase Fluorescence In Situ Hybridization (FISH).. Shiraz E-Med J. 2009;10(4):. doi:
23
Jan
2016
[Retracted Article] Methylation of the Wnt Signaling Antagonist, Wnt Inhibitory Factor 1 and Dickkopf-1 Genes in Acute Myeloid Leukemia at the Time of Diagnosis
Ali Ghasemi,
Abbas Ghotaslou,
Mohsen Mohammadi,
Sadegh Abbasian,
Kazem Ghaffari
Ghasemi A, Ghotaslou A, Mohammadi M, Abbasian S, Ghaffari K. [Retracted Article] Methylation of the Wnt Signaling Antagonist, Wnt Inhibitory Factor 1 and Dickkopf-1 Genes in Acute Myeloid Leukemia at the Time of Diagnosis. Zahedan J Res Med Sci. 2016;18(1):e5874. doi: https://doi.org/10.17795/zjrms-5874
Crossmark
Checking
Comments
Number of Comments:0Cited by
- Scopus by DOI: 0
Last Update: 1 month ago
- Scopus by Title: 0
Last Update: 1 month ago
- Scopus by Title (Ref): 0
Last Update: 1 month ago
- CrossRef: 0
Last Update: 4 hours ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Search Relations
Author(s):
Related Articles