Investigation between Alcoholic Extract and Essential Oil Melissa Officinalis L. New in Growth Inhibition of E. coli

Background: Therapy of plant is not any side effects and drug resustant for inhibition of
disease in world. In this study, antimicrobial effects of Melissa officinalis L. ethanol
exteract and essential oil on E. coli have been investigated.
Materials and Methods: In this investigation, diameter of inhibitory zone of these
materials was measured in disk agar diffusion method. Therefore, Melissa officinalisL.
ethanolic exteract (80%) and essential oil have been supplied. Then, antimicrobial activity
these substances next for 24 hour for ethanol extraction 80% concentration 50 to 1000
mg/mL and essential oil concentration with 3% to 100% has been considered. Also,
comparison of mean diameter of inhibitory zone between treatment and control groups of
ANOVA has been used.
Results: Results of these investigations were shown that extract ethanol Melissa officinalis
L. was any inhibitory effect on E. coli growth 24 hour after of treatment. Also, diameter of
inhibitory zone for 100% essential oil have been 33.2±0.13mm in E. coli that has been
increased in comparison control groups significantly (p=0.001). This inhibitory effects was
more than cefixime and cefteriaxone (p=0.00, p=0.01).
Conclusion: These found were shown that this plant prevents growth of E. coli In vitro
condition. This essential oil could be suggested as antimicrobial agents for inhibition of
bacterial diseases in human.