The inhibitory effect of low frequency electromagnetic field (50Hz) on angiogenesis in chorioalantoic membrane of chick

Background: Inhibition of angiogenesis is a major target in the fight against cancer and other diseases.  The effects of electromagnetic fields on cell development and growth have been investigated. In this research we investigated the effects of electromagnetic fields with 100, 200 & 300 gauss on angiogenesis in chorioalantoic membrane of chick embryos. 

Materials and Methods: Ross fertilized eggs were used that divided in 4 random groups: 1)control, 2)experimental 1, treated with 100G electromagnetic field 3)experimental 2, treated with 200G electromagnetic field and 4)experimental 3, treated with 300G electromagnetic field.  In day 2 of incubation a window was opened on eggs in sterile condition, then in day 8 a gelatin sponge was occurred on chorioalantoic membrane and was soaked with 5 µl FCS.  Members of groups 2 were transferred into electromagnetic field (100G), group 3(200G) and group 4(300G) in day 10. In 12^th day all cases were photographed by photo-stereomicroscope. The numbers and lengths of vessels around the sponges were measured and compared with each other by t-test & ANOVA (p<0.05).    

Results: In this study we reported that, no significant vascular reaction was detectable 3 hr exposure to 100,300G electro magnetic fields did not affect on angiogenesis but 200G electromagnetic field inhibited angiogenesis in chorioalantoic membrane.    

Conclusion: For this reasons absence of adverse effects on embryo development and absence of mutagenic effects, exposure to 200G electromagnetic field could be proposed as a safe and cost-effective treatment of human diseases involving excessive angiogenesis.