Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Hadi Hasanzadeh; Mostafa  Rezaei Tavirani; Samaneh Sadat Seyyedi; Hakimeh Zali; Saeid Heydari Keshel; Majid Jadidi; Ali Abedelahi

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis

Author(s):

Hadi Hasanzadeh¹,

Mostafa Rezaei Tavirani^2,*,

Samaneh Sadat Seyyedi³,

Hakimeh Zali⁴,

Saeid Heydari Keshel⁴,

Majid Jadidi¹,

Ali Abedelahi⁵

1Dept. of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran

2Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Dept. of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran

4Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5Dept. of Anatomy, Tabriz University of Medical Sciences, Tabriz, Iran

International Journal of Cancer Management:Vol. 7, issue 1; e80504

Published online:Mar 31, 2014

Article type:Research Article

Received:Jun 23, 2013

Accepted:Aug 30, 2013

How to Cite:Hadi HasanzadehMostafa Rezaei TaviraniSamaneh Sadat SeyyediHakimeh ZaliSaeid Heydari KeshelMajid JadidiAli Abedelahiet al.Effect of ELF-EMF Exposure on Human Neuroblastoma Cell Line: a Proteomics Analysis.Int J Cancer Manag.2014;7(1):e80504.

Abstract

Background: Extremely low frequency electromagnetic fields (ELF-EMF) have been common in daily life all over the world. They have produced by power lines and electrical appliances, but higher levels of them have raised a lot of concerns about their carcinogenesis. Both epidemiological and laboratory studies have suggested that EMFs might increase cancer incidence, including acute childhood leukemia, brain and breast cancer.

Methods: In the present study, SH-SY5Y human neuroblastoma cell line has exposed to 2mT, 50 Hz magnetic field for 3 h. Next, effect of this exposure on protein expression including over-expression or under-expression has assessed by proteomics.

Results: Bioinformatics and statistical analysis using progenesis same spot software on the obtained 2D electrophoresis has shown that expression of 189 proteins in exposed group has changed relative to control. Besides, PCA analysis has verified results of clustering, and has shown that protein data has clustered according to experimental conditions.

Conclusion: The results of this study have shown that ELF-EMF changes cell morphology via altering protein expression, but more profound studies have needed to determine the kind of proteins altered.

Keywords

Electromagnetic fields

Proteomics

Neuroblastoma

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