Determination of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC

authors:

avatar Somayeh Esmaeili 1 , avatar Farzaneh Naghibi 1 , * , avatar Mahmoud Mosaddegh 1 , avatar Nazli Nader 1

Traditional Medicine & Materia Medica Research Center, School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services, Tehran, Iran

how to cite: Esmaeili S, Naghibi F, Mosaddegh M, Nader N. Determination of 18 β-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC. Iran J Pharm Res. 2006;5(2):e128276. https://doi.org/10.22037/ijpr.2010.666.

Abstract

A high performance liquid chromatography method was studied for determination of 18 β-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and UV detection at 230 nm. The recoveries were %99.60-%102.81 with relative standard deviation between %0.01-%1.58. The relative standard deviation of the repeatability test was %2.96. The method is simple, rapid, safe, accurate, economical and useful for standardization of the licorice products.