Biological and immunological characteristics of Brucella abortus S99 major outer membrane proteins

Seyed Davar Siadat; Mohammad Reza Aghasadeghi; Sahar Karami; Seyed Mehdi Sadat; Arfa Moshiri

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

Current Issue All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

Biological and immunological characteristics of Brucella abortus S99 major outer membrane proteins

Author(s):

Seyed Davar Siadat^1,*,

Mohammad Reza Aghasadeghi²,

Sahar Karami²,

Seyed Mehdi Sadat³,

Arfa Moshiri²

1Department of Bacterial Vaccines and Antigens Production, and Department of Hepatitis and AIDS, Pasteur Institute of Iran, [email protected], Iran

2Department of Hepatitis and AIDS, Pasteur Institute of Iran, Iran

3Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Karaj Branch, Iran

Jundishapur Journal of Microbiology:Vol. 4, issue 1

Published online:Jan 31, 2011

Article type:Research Article

How to Cite:Siadat SD, Aghasadeghi MR, Karami S, Sadat SM, Moshiri A. Biological and immunological characteristics of Brucella abortus S99 major outer membrane proteins. Jundishapur J Microbiol. 2011;4(1):. doi:

Abstract

Introduction and objective: Outer membrane proteins (OMPs) of Brucella are considered as immunogenic structures which can be used to design and develop a subunit vaccine for human brucellosis. Brucella abortus S99 OMPs promote the synthesis of high levels of specific anti-Brucella IgG molecules in rabbits when administrated with lipopolysaccharide (LPS). The objective of this study is evaluation of the efficacy of B. abortus major OMPs with LPS in the induction of immune response against brucellosis.

Materials and methods: OMPs were derived from B. abortus by sequential extraction of sonicated cells with ultracentrifugation and predigestion with lysozyme. Proteins could be separated by anion-exchange chromatography and gel-filtration. Based on SDS-PAGE profiles, porins have been dominantly purified among three different classes of B. abortus OMPs. Sera of immunized rabbits against B. abortus porins were analyzed by enzyme-linked immunosorbent assay (ELISA). LPS of B. abortus and complete Freud's adjuvant (CFA) were also applied to elicit higher levels of anti-Brucella antibodies.

Results: ELISA confirmed the potency of porins and porins combination with CFA and LPS to promote humoral specific response. Among the above-mentioned compounds, a combination of porins + LPS or porins + CFA has been the most potent immunogenic compound to induce higher titer of antibody against B. abortus S99 in the animal model.

Conclusion: The application of a complex of Brucella LPS and porins as an effective method to elicit protective and long-lasting immunity against Brucella infection and would be studied to design and develop a subunit vaccine for human brucellosis.

Keywords

Brucella abortus S99

Outer membrane proteins

Porins

Lipopolysaccharide

Full Text

Full text is available in PDF

Comparison of Biological and Immunological Characterization of Lipopolysaccharides From Brucella abortus RB51 and S19

Zahra Kianmehr,

Sussan Kaboudanian Ardestani,

Hoorieh Soleimanjahi,

Fatemeh Fotouhi,

Saeed Alamian,

Shahin Ahmadian

Kianmehr Z, Kaboudanian Ardestani S, Soleimanjahi H, Fotouhi F, Alamian S, et al. Comparison of Biological and Immunological Characterization of Lipopolysaccharides From Brucella abortus RB51 and S19. Jundishapur J Microbiol. 2015;8(11):e24853. doi: https://doi.org/10.5812/jjm.24853

19

Jun

2003

Identification of Brucella abortus (S19) immunogens using two- dimensional gel electrophoresis and westernblotting

J Abdolalizadeh,

A Mostafaie,

H Kharazi,

B Nomanpour,

H Nomani

Abdolalizadeh J, Mostafaie A, Kharazi H, Nomanpour B, Nomani H. Identification of Brucella abortus (S19) immunogens using two- dimensional gel electrophoresis and westernblotting. J Kermanshah Univ Med Sci. 2003;7(1):e81199. doi:

14

Nov

2017

A Recombinant Chimera Protein as a Novel Brucella Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice

Abbas Abdollahi,

Shahla Mansouri,

Jafar Amani,

Mahdi Fasihi-Ramandi,

Reza Ranjbar,

Amir Ghasemi

,et al.

Abdollahi A, Mansouri S, Amani J, Fasihi-Ramandi M, Ranjbar R, et al. A Recombinant Chimera Protein as a Novel Brucella Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice. Jundishapur J Microbiol. 2018;11(1):e12776. doi: https://doi.org/10.5812/jjm.12776

1

Sep

2013

Optimization and Efficient Purification in Production of Brucella melitensis Recombinant HSP and TF Proteins With Low Endotoxin Contents

Amir Ghasemi,

Mohammad Hossein Salari,

Mohammad Reza Pourmand,

Amir Hassan Zarnani,

Hojat Ahmadi,

Mohamad Hassan Shirazi

,et al.

Ghasemi A, Salari MH, Pourmand MR, Zarnani AH, Ahmadi H, et al. Optimization and Efficient Purification in Production of Brucella melitensis Recombinant HSP and TF Proteins With Low Endotoxin Contents. Jundishapur J Microbiol. 2013;6(7):e6875. doi: https://doi.org/10.5812/jjm.6875

1

Sep

2023

Genetic manipulation of probiotic bacterium Lactococcus lactis to produce OMP10 protein of Brucella abortus

kazemi kazemi,

Abbas Doosti,

Mohammad Saeid Jami

kazemi K, Doosti A, Jami MS. Genetic manipulation of probiotic bacterium Lactococcus lactis to produce OMP10 protein of Brucella abortus. koomesh. 2023;25(3):e152826. doi:

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 1 week ago
Scopus by Title: 5
Last Update: 1 week ago
Scopus by Title (Ref): 5
Last Update: 1 week ago
CrossRef: 0
Last Update: 6 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Seyed Davar Siadat:[PubMed][Scholar]
Mohammad Reza Aghasadeghi:[PubMed][Scholar]
Sahar Karami:[PubMed][Scholar]
Seyed Mehdi Sadat:[PubMed][Scholar]
Arfa Moshiri:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

Outlines

Biological and immunological characteristics of Brucella abortus S99 major outer membrane proteins

Abstract

Full Text

Similar Articles

Comparison of Biological and Immunological Characterization of Lipopolysaccharides From Brucella abortus RB51 and S19

Identification of Brucella abortus (S19) immunogens using two- dimensional gel electrophoresis and westernblotting

A Recombinant Chimera Protein as a Novel Brucella Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice

Optimization and Efficient Purification in Production of Brucella melitensis Recombinant HSP and TF Proteins With Low Endotoxin Contents

Genetic manipulation of probiotic bacterium Lactococcus lactis to produce OMP10 protein of Brucella abortus

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 6 days ago