Cloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli

Hamid Galehdari; Sara Alaee; Maisam Mirzaee

Jundishapur Journal of Microbiology

Ahvaz Jundishapur University of Medical Sciences

Current Issue All Issues In Press Accepted Manuscripts Search

Editors & Boards Journal Information Indexing and Listing Sources Journal Metrics Reviewer and AE Registration Form Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support

APC

Authors Guide Submit Manuscript

Cloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli

Author(s):

Hamid Galehdari^1,*,

Sara Alaee²,

Maisam Mirzaee²

1Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, [email protected], Iran

2Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Iran

Jundishapur Journal of Microbiology:Vol. 2, issue 1; 31-35

Article type:Research Article

Received:Sep 01, 2008

Accepted:Mar 01, 2009

How to Cite:Hamid GalehdariSara AlaeeMaisam MirzaeeCloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli.Jundishapur J Microbiol.2(1):31-35.

Abstract

The Azotobacter vinelandii is an aerobic, nitrogen-fixing bacterium with the ability to accumulate polyesters such as poly (3-hydroxybutyrate), a particular group of the polyhydroxyalkanoates (pha), when will be cultivated on several carbon sources, including sucrose. Although the capacity of Azotobacter strains to accumulate polyesters is well known, and the genes responsible for their synthesis have recently been identified. However, high production costs have hindered the use of polyhydroxyalkanoates as bioplastics, since their final price is considerably high. The phb gene clusters including phbBAC genes can alternatively be cloned and expressed in the Escherichia coli with the goal to produce high cell density cultivation. We amplified separately the phbB, phbA and phbC genes from extracted Azotobacter genome with the designed primers and could clone the fragments in the cloning vector pCR 2.1- TOPO with the subsequent transformation of E. coli strain DH5α.

Keywords

Azotobacter vinelandii

Recombinant Escherichia coli

phb gene cluster

poly (3-hydroxybutyrate)

Full Text

Full text is available in PDF

comments

Leave a comment here

Alice

Jun 30, 2022

Interestingly Information

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:1

Metrics

Purchasing Reprints

Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here ( https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.

Search Relations

Author(s):

Hamid Galehdari:[PubMed][Scholar]
Sara Alaee:[PubMed][Scholar]
Maisam Mirzaee:[PubMed][Scholar]