First Survey of Metallo-β–Lactamase Producers in Clinical Isolates of Pseudomonas aeruginosa From a Referral Burn Center in Kurdistan Province

Enayatollah kalantar; Vahideh Torabi; Heiman Salimizand; Fariborz Soheili; Soheila Beiranvand; Mohammad Mehdi Soltan Dallal

doi:10.17795/jjnpp-3546

Jundishapur Journal of Natural Pharmaceutical Products

The Official Journal of Ahvaz Jundishapur University of Medical Sciences

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https://doi.org/10.17795/jjnpp-3546

First Survey of Metallo-β–Lactamase Producers in Clinical Isolates of Pseudomonas aeruginosa From a Referral Burn Center in Kurdistan Province

Author(s):

Enayatollah kalantar^1,*,

Vahideh Torabi²,

Heiman Salimizand³,

Fariborz Soheili⁴,

Soheila Beiranvand⁵,

Mohammad Mehdi Soltan Dallal⁶

1Envirronmental Health Resaerch Center, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

2Department of Biological Sciences, Islamic Azad University, Sciences Research Branch, Tehran, IR Iran

3Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

4School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

5Qods Hospital, Kurdistan University of Medical Sciences, Sanandaj, IR Iran

6Division of Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran

Jundishapur Journal of Natural Pharmaceutical Products:Vol. 7, issue 1; e94148

Published online:Jan 04, 2012

Article type:Research Article

Received:May 20, 2019

Accepted:Dec 05, 2011

How to Cite:Enayatollah kalantarVahideh TorabiHeiman SalimizandFariborz SoheiliSoheila BeiranvandMohammad Mehdi Soltan Dallalet al.First Survey of Metallo-β–Lactamase Producers in Clinical Isolates of Pseudomonas aeruginosa From a Referral Burn Center in Kurdistan Province.Jundishapur J Nat Pharm Prod.7(1):e94148.https://doi.org/10.17795/jjnpp-3546.

Abstract

Background: Treatment of infectious diseases is becoming more challenging with each passing year. This is especially true for infections caused by Pseudomonas aeruginosa, an opportunistic pathogen with the ability to rapidly develop resistance to multiple classes of antibiotics.

Objectives: This study was conducted to determine the prevalence of metallo-β-lactamase (MBL)–producing strains among multidrug-resistant P. aeruginosa strains isolated from burn patients.

Materials and Methods: The isolates were identified, tested for susceptibility to various antimicrobial agents, and screened for the presence of MβLs by using the double-disk synergy test. The minimal inhibitory concentration of imipenem was determined by microplate broth dilution method on Mueller-Hinton agar. To detect VIM, SIM, and GIM MBLs, the isolates were subjected to polymerase chain reaction.

Results: In this study, we identified 100 P. aeruginosa isolates from 176 clinical specimens obtained from burn patients. The isolates showed maximum resistance to ampicillin (100%), ceftazidime (94%), and ceftriaxone (89%). The CLSI-MBL phenotypic test showed that of the 100 P. aeruginosa isolates, 22 (22%) were positive for MBL production in the double-disk synergy test. Of the 22 MBL-positive P. aeruginosa isolates, 8 were resistant to imipenem. PCR analysis showed that 8 isolates were positive for blaVIM1. The other genes blaSIM1 and blaGIM1 were not detected.

Conclusions: The study results demonstrate the serious therapeutic threat of the spread of MBL producers among P. aeruginosa populations. Metallo-β-lactamases were detected in 22% of imipenem-resistant P. aeruginosa isolates. Early detection and infection-control practices are the best antimicrobial strategies for this organism; therefore, systematic surveillance to detect MBL producers is necessary.

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Author(s):

Enayatollah kalantar:[PubMed][Scholar]
Vahideh Torabi:[PubMed][Scholar]
Heiman Salimizand:[PubMed][Scholar]
Fariborz Soheili:[PubMed][Scholar]
Soheila Beiranvand:[PubMed][Scholar]
Mohammad Mehdi Soltan Dallal:[PubMed][Scholar]