Effects of Raloxifene and GnRH-a on VEGF expression in cultured human uterine leiomyoma cells

Taravat Fakheri; Kamran Mansouri; Sahar Rashidi; Mansour Rezaei; Sara Daeichin; Anisodowleh Nankali

doi:10.22110/jkums.v18i2.1552

Journal of Kermanshah University of Medical Sciences

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https://doi.org/10.22110/jkums.v18i2.1552

Effects of Raloxifene and GnRH-a on VEGF expression in cultured human uterine leiomyoma cells

Author(s):

Taravat Fakheri¹,

Kamran Mansouri²,

Sahar Rashidi^1,*,

Mansour Rezaei³,

Sara Daeichin¹,

Anisodowleh Nankali¹

1Maternity Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Social Development and Health Promotion Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 18, issue 2; e74164

Published online:May 29, 2014

Article type:Research Article

Received:Oct 05, 2013

Accepted:Mar 11, 2014

How to Cite:Taravat FakheriKamran MansouriSahar RashidiMansour RezaeiSara DaeichinAnisodowleh Nankaliet al.Effects of Raloxifene and GnRH-a on VEGF expression in cultured human uterine leiomyoma cells.J Kermanshah Univ Med Sci.18(2):e74164.https://doi.org/10.22110/jkums.v18i2.1552.

Abstract

Background: uterine leiomyoma is the most common benign pelvic tumor in women of reproductive age and is frequently associated with abnormal uterine bleeding and pelvic pressure. Combined therapy of gonadotropin-releasing hormone analogue (GnRH-a) and selective estrogen receptor inhibitors (raloxifene) reduce the size of leiomyoma.

Methods: Twelve uterine leiomyomas were obtained from pre-menopausal women with regular menstrual cycles in the proliferative phase who underwent abdominal hysterectomy or myomectomy in Imam Reza Hospital. The patients had received no hormonal therapy for at least 6 months before surgery.The comparative effects of treatment with graded concentrations of raloxifene (in five groups with various concentrations of 1, 2, 4, 6 and 8 micrograms per milliliter, respectively) and GnRH-a (in five groups, with concentrations of 50, 100, 200, 300 and 400 micrograms per milliliter, respectively) and combination of these two on Vascular Endothelial Growth Factor (VEGF) and inhibition of leiomyoma cell proliferation were determined.

Results: in the three treatment groups with graded concentration of raloxifene and GnRH-a and combined therapy, VEGF content significantly decreased (P<0.05). The VEGF decreasing levels in the combined therapy, raloxifene and GnRH-a groups were 79.16%, 64.74% and 55.29%, respectively (p<0.05). The inhibition of cell growth was significantly different between the two treatment groups (P<0.05). The inhibition of cell growth in the combined therapy group was 70.77% followed by raloxifene (60.76%) and GnRH-a (15.83%), which indicated a significant difference (P<0.05).

Conclusion: Treatment with graded concentrations of Raloxifene, GnRH-a and combination of both Raloxifene and GnRH-a significantly decreased the VEGF content and proliferation of leiomyoma cells.

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Author(s):

Taravat Fakheri:[PubMed][Scholar]
Kamran Mansouri:[PubMed][Scholar]
Sahar Rashidi:[PubMed][Scholar]
Mansour Rezaei:[PubMed][Scholar]
Sara Daeichin:[PubMed][Scholar]
Anisodowleh Nankali:[PubMed][Scholar]