Comparison of anti-oxidant enzymes activity and levels of zinc and selenium in sperm and seminal plasma between fertile and idiopathic infertile men

Hadi Kharazi; Asad Vaisi Raigani; Behrouz Etesami; Mozafar Khazaei; Amir Kiani; Ezatollah Rafiee Alavi; Sayyed-Saeed Shahrokhi

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Comparison of anti-oxidant enzymes activity and levels of zinc and selenium in sperm and seminal plasma between fertile and idiopathic infertile men

Author(s):

Hadi Kharazi¹,

Asad Vaisi Raigani²,

Behrouz Etesami^3,*,

Mozafar Khazaei²,

Amir Kiani⁴,

Ezatollah Rafiee Alavi⁵,

Sayyed-Saeed Shahrokhi⁶

1Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Studenr Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

4Dept. of Toxicology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

5Dept. of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran

6Dept. of Urology, School of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 14, issue 4; e79445

Published online:Mar 19, 2011

Article type:Research Article

Received:Dec 01, 2009

Accepted:May 25, 2010

How to Cite:Hadi KharaziAsad Vaisi RaiganiBehrouz EtesamiMozafar KhazaeiAmir KianiEzatollah Rafiee AlaviSayyed-Saeed Shahrokhiet al.Comparison of anti-oxidant enzymes activity and levels of zinc and selenium in sperm and seminal plasma between fertile and idiopathic infertile men.J Kermanshah Univ Med Sci.14(4):e79445.

Abstract

Background: Reactive oxygen species (ROS)-induced lipidperoxidation can lead to dysfunction of sperm and thereby, infertility may be occurred. So, always there is a balance between amount of ROS and anti-oxidant molecules in semen. Anti-oxidant enzymes of sperm; superoxide dismutase (SOD), glutathione peroxidase (GPX), catalse and zinc and selenium can protect it from destructive effects of ROS. Hence, the present study was designed to compare the activities of these enzymes and trace elements between fertile and idiopathic infertile men.

Methods: Semen specimens were collected from 30 infertile men with proven infertility by an urologist, and 30 fertile men as control donors, with age range between 20-40 years old. Semen analysis was conducted by CASA method. Atomic absorption method was used for measuring of zinc and selenium concentration. Activity assays of SOD and GPX were performed by Randox Kits. Aebi method also was applied for evaluation of catalase activity.

Results: There was no difference between the activities of enzymes in fertile men and infertile ones. Also, it wasn't seen any difference in the selenium and zinc levels of seminal plasma. There was no relationship between evaluated items with sperm parameters. Only, in asthenoteratospermic individuals negative correlations were found between GPX and sperm motility, selenium and sperm morphology. Also, in these individuals ,there was a positive correlation between SOD and catalse activity.

Conclusion: Measuring activities of SOD, GPx, and catalase and the contents of zinc and selenium of seminal plasma do not appear to be suitable tools for determining the fertility potential of sperm.

Keywords

idiopathic infertility

anti-oxidant enzyme

zinc

selenium

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