Comparison of anti-oxidant enzymes activity and levels of zinc and selenium in sperm and seminal plasma between fertile and idiopathic infertile men

authors:

avatar Hadi Kharazi 1 , avatar Asad Vaisi Raigani 2 , avatar Behrouz Etesami 3 , * , avatar Mozafar Khazaei 2 , avatar Amir Kiani 4 , avatar Ezatollah Rafiee Alavi 5 , avatar Sayyed-Saeed Shahrokhi 6

Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Studenr Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Dept. of Toxicology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Dept. of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran
Dept. of Urology, School of Medicine, Lorestan University of Medical Sciences, Khorram Abad, Iran
Journal of Kermanshah University of Medical Sciences: Vol.14, issue 4; e79445
published online: March 19, 2011
article type: Research Article
received: December 01, 2009
accepted: May 25, 2010

how to cite: Kharazi H, Vaisi Raigani A, Etesami B, Khazaei M, Kiani A, et al. Comparison of anti-oxidant enzymes activity and levels of zinc and selenium in sperm and seminal plasma between fertile and idiopathic infertile men. J Kermanshah Univ Med Sci. 2011;14(4):e79445. 

Abstract

Background: Reactive oxygen species (ROS)-induced lipidperoxidation can lead to dysfunction of sperm and thereby, infertility may be occurred. So, always there is a balance between amount of ROS and anti-oxidant molecules in semen. Anti-oxidant enzymes of sperm; superoxide dismutase (SOD), glutathione peroxidase (GPX), catalse and zinc and selenium can protect it from destructive effects of ROS. Hence, the present study was designed to compare the activities of these enzymes and trace elements between fertile and idiopathic infertile men.
Methods: Semen specimens were collected from 30 infertile men with proven infertility by an urologist, and 30 fertile men as control donors, with age range between 20-40 years old. Semen analysis was conducted by CASA method. Atomic absorption method was used for measuring of zinc and selenium concentration. Activity assays of SOD and GPX were performed by Randox Kits. Aebi method also was applied for evaluation of catalase activity.
Results: There was no difference between the activities of enzymes in fertile men and infertile ones. Also, it wasn't seen any difference in the selenium and zinc levels of seminal plasma. There was no relationship between evaluated items with sperm parameters. Only, in asthenoteratospermic individuals negative correlations were found between GPX and sperm motility, selenium and sperm morphology. Also, in these individuals ,there was a positive correlation between SOD and catalse activity.
Conclusion: Measuring activities of SOD, GPx, and catalase and the contents of zinc and selenium of seminal plasma do not appear to be suitable tools for determining the fertility potential of sperm.

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