Molecular analysis of alpha globin gene deletions among patients with microcytic hypochromic anemia in Kermanshah-Iran

authors:

avatar Reza Alibakhshi 1 , * , avatar Majid Arash 2 , avatar Reza Akramipour 3 , avatar Hamid Nomani 1 , avatar Mohammad-Reza Farshchi 4 , avatar Soheila Fathollahi 4 , avatar Mansour Rezaei 5

Dept. of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Studenr Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Dept. of Pediatric, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Central Laboratory of Medical Genetic, Kermanshah University of Medical Sciences, Kermanshah, Iran
Dept. of Biostatistics, School of Medicine and Health Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Journal of Kermanshah University of Medical Sciences: Vol.14, issue 4; e79447
published online: March 19, 2011
article type: Research Article
received: January 09, 2010
accepted: June 08, 2010

how to cite: Alibakhshi R, Arash M, Akramipour R, Nomani H, Farshchi M, et al. Molecular analysis of alpha globin gene deletions among patients with microcytic hypochromic anemia in Kermanshah-Iran. J Kermanshah Univ Med Sci. 2011;14(4):e79447. 

Abstract

Background: The majority of α-thalassemi mutations are deletions of one or both α-globin genes. Since the Iranian populaion is a mixture of different ethnic groups, frequency and distribution of globin mutations in various regions of the country need to be clarified. The aim of this study was to determine the common alpha globin gene deletions among individuals with hypochromic microcytic anemia in Kermanshah province.
Methods: Following the initial evaluation, 92 patients (47 women and 45 men) were found as microcytic hypochromic [MCV < 80 fl and MCH< 27 pg] anemia and selected for this study. All samples were analyzed for detection of four α-gene deletions (-α3.7,-α4.2,-(α) 20.5 and --MED) by GAP-PCR technique. After amplification, 10µl of PCR product was electrophoresed through 1.2% agarose gel and bands were visualized by staining gel in ethidium bromide solution and photographed under a UV transilluminater.
Results: 45 patients had -α3.7 single gene deletion. In patients with -α3.7 deletion, in both homozygous and heterozygous states, MCH was lower than normal ranges. However, the percent of  HbA2 was in normal range. In this study, other common deletional mutations, including - (α)20.5, -α4.2 and --MED were not found.
Conclusion: The results of persent study showed that the frequency of -α3.7 single gene deletion among patients with microcytic hypochromic anemia in Kermanshah province was 48.9%.

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