Effects of synthesized iron oxide nanoparticles from Ziziphora clinopodioides on expression of the efflux pump genes of Staphylococcus aureus

authors:

avatar Mostafa Sajadian , avatar maryam teimouri , *

Koomesh: Vol.22, issue 3; 542-549
article type: issue_documents_importer
received: August 01, 2019
accepted: November 02, 2019

how to cite: Sajadian M, teimouri M. Effects of synthesized iron oxide nanoparticles from Ziziphora clinopodioides on expression of the efflux pump genes of Staphylococcus aureus. koomesh. 2020;22(3):e153212. 

Abstract

Introduction: One of the important factors causing nosocomial infections is drug-resistant Staphylococcus aureus strain, so correspondingly, having norA efflux pump is one of the bacterial;#39s ability to generate drug resistance. Iron has been used as a nanoparticle with antimicrobial activity against bacteria. The aim of this study was to investigate the effect of iron oxide nanoparticles synthesized from ziziphora plant extract on Staphylococcus aureus efflux pump genes expression. Materials and Methods: green iron nanoparticles were formed using ethanolic extract of ziziphora plant and iron salt solution and identified by SEM and TEM electron microscopy. The Staphylococcus aureus strains were collected and isolated from hospitals and laboratories in Tehran. Considerably, Antimicrobial activity of green nanoparticles against strains was investigated. DNA and RNA and cDNA were extracted and the relative expression of norA gene in Staphylococcus aureus isolates was evaluated by Real-time PCR method. Results: Different strains, under the influence of iron nanoparticles, showed different expression changes That were statistically significant compared to gmk gene expression (P

References

  • 1.

    Parchagani F. Iron oxide nanoparticles: Synthesis, characterization and investigation of catalytic properties [dissertation]. Zanjan Univ 2012. (Persian).

  • 2.

    Hadadi T, Najafi M A. Green synthesis and characterization of iron oxide nanoparticles using Cinnamon medicinal herb. proceedings of the third international and the Sixth national conference of medicinal herbs and stable agriculture; Zabol, Iran. Hamedan Conf Paper 2017. (Persian).

  • 3.

    Gupta D, Singh A, U. Khan A. Nanoparticles as efflux pump and biofilm inhibitor to rejuvenate bactericidal effect of conventional antibiotics. Nanoscale Res Lett 2017; 12: 454.

  • 4.

    Fazlzadeh M, Tagizadeh A, Entezari A, Khosravi R. Green synthesis of ZnO nanoparticles and coating them on the activated carbon to investigate removal efficiency of hexavalent chromium. Occup Environ Health 2017; 3: 7-19. (Persian).

  • 5.

    Zare E, Pourseyedi Sh, Darezereshki E, Pardakhti A. Green synthesis of Iron oxide nanoparticles using rosemary plant leaf extract. proceedings of the first electronic conference on new findings in the environment and agricultural ecosystems; 2014 Nov 22; Kerman Shahid Bahonar university, Iran. Univ Tehran CIVILICA 2014. (Persian).

  • 6.

    Manikandan V, Madhu GC, Pavithra V, Jaianand K, Balaji P. Green synthesis, characterization of Iron oxide nanoparticles using leucas aspera leaf extract and evaluation of antibacterial and antioxidant studies. Int J Agricul Innovat Res 2017; 6: 242-250.

  • 7.

    Kanagasubbulakshmi S, Kadirvelu K. Green synthesis of Iron Oxide nanoparticles using lagenaria siceraria and evaluation of its antimicrobial activity. Def Life Sci J 2017; 2: 422-427.

  • 8.

    Kazemi SS, Nemati Mansoor F, Mirzaie A, Ashrafi F. Antibiotic resistance assessment, and genotypic and phenotypic detection of norA efflux pump in methicillin and ciprofloxacin resistant Staphylococcus aureus isolates. J Microbial World 2017; 9: 286-296. (Persian).

  • 9.

    Ezati S, Mirzaie A, Zandi M. Evaluation of anti-efflux activity of anthemis atropatana extract against ciprofloxacin resistant staphylococcus aureus Isolates. JMP 2018; 2: 122-134. (Persian).

  • 10.

    Nejabat dust A. Effect of zinc oxide nanoparticles functionalized with carbazone thiosemia on norA gene expression pattern Hospital Pathogen Staphylococcus aureus Bacteria. [dissertation]. Rasht Islamic Azad Univ 2017. (Persian).

  • 11.

    Yoshida H, Bogaki M, Nakamura S, Ubukata K, Konno M. Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones. J Bacteriol 1990; 172: 6942-6949.

  • 12.

    Rajabian M, Bonyadian M, Abbasvali M, Khanjari A. Investigation on the antibacterial properties of ziziphora cliniopodiodes and thymus daenensis essential oils against some food pathogenic bacteria. J Appl Microbiol Food Indust 2017; 3: 65. (Persian).

  • 13.

    Shafei M, Sharifan A, Aghazade Meshki M. Composition of essential oil of ziziphora clinopodioides and its antimicrobial activity on kluyveromyces marxianus. Food Technol Nutr 2012; 9: 101-108. (Persian).

  • 14.

    Teimouri M. Antimicrobial effects of Teucrium polium L. alcoholic extract on Gram positive and negative bacteria. J Biol Findings 2011; 8: 1-6. (Persian).

  • 15.

    Mirzaie A, Noorbazargan H, Rahmati H, Zandi M. A study of gene expression and activity of NorA efflux pump in clinical isolates of ciprofloxacin resistant staphylococcus aureus. JBUMS 2016; 18: 63-70. (Persian).

  • 16.

    Haddadi Zahmatkesh M, Laripoor M, Mirzaie A & Ashrafi F. Prevalence of norA and norB efflux pump genes in clinical isolates of Staphylococcus aureus and their contribution in ciprofloxacin resistance. Iran J Med Microbiol 2016; 10: 20-30.

  • 17.

    Mirzaie A, Bagheri Kashtali AA, Noorbazargan H, Sadat Shandiz SA. The inhibitory effect of artemisa quttensis extract on norA efflux pump in ciprofloxacin resistant staphylococcus aureus strains using ethidium bromide and real-time PCR methods. Qom Univ Med Sci J 2017; 11: 51-63. (Persian).

  • 18.

    Chitsaz M, Pargar A, Naseri M, Kamalinejhad M, Bazargan M, Mansuri S, et al. Composition of essential oil and antibacterial effects of hydroalcoholic extract and essential oil of the plant ziziphora clinopodiodes: LAM on Selected Bacteria. Daneshvar Med 2007; 14: 15-22. (Persian).

  • 19.

    Ozturk S, Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora cliniopodioides. Food Control 2007; 18: 535-540.

  • 20.

    Salehi P, Sonboli A, Eftekhar F, Nejadebrahimi S, Yousefzadi M. Essential oil composition, antibacterial and antioxidant activity of the oil and various extracts of Ziziphora cliniopodioides subsp. rigida (BOISS.) RECH. f. from Iran. Biol Pharm Bull 2005; 28: 1892-1896. (Persian).

  • 21.

    Goudarzi H, Doraghi M, Dabiri H, Ghalavand Z. Functional analysis of multidrug efflux pumps genes of Acinetobacter baumannii strains. Res Medicine 2013; 37: 107-112. (Persian).