Antibacterial Activity of Essential Oils and Plant Extracts of Artemisia (Artemisia annua L.) In Vitro

authors:

avatar Alireza Massiha 1 , * , avatar Mohammadreza Majid Khoshkholgh-Pahlaviani 2 , avatar Khosro Issazadeh 1 , avatar Sirus Bidarigh, 3 , avatar Saeed Zarrabi 4

Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Lahijan, Iran
Department of Biotechnology, Faculty of Basic Sciences, Islamic Azad University, Lahijan, Iran
Department of Agriculture, Faculty of Natural Resource, Islamic Azad University, Lahijan, Iran
Department of Chemistry, Faculty of Basic Sciences, Islamic Azad University, Lahijan, Iran
Zahedan Journal of Research in Medical Sciences: Vol.15, issue 6; e92933
published online: October 29, 2012
article type: Research Article
received: July 21, 2011
accepted: May 06, 2011

how to cite: Massiha A , Majid Khoshkholgh-Pahlaviani M, Issazadeh K, Bidarigh, S, Zarrabi S. Antibacterial Activity of Essential Oils and Plant Extracts of Artemisia (Artemisia annua L.) In Vitro. Zahedan J Res Med Sci. 2013;15(6):e92933. 

Abstract

Background: Many of the plants used to treat certain diseases, because they have showed antimicrobial activity. In this case, many studies have conducted on antimicrobial and antioxidant activity of Artemisia annua.
Materials and Methods: The purpose of this study is to determine the antibacterial effects of aqueous, chloroform, methanol and ethanol extracts of A. annua against eight bacterial species. Antimicrobial activity, minimum inhibitory concentration and minimum bactericidal activity of the essential oil and extract was performed by agar disc diffusion and microdilution broth methods6T.
Results: The obtained results showed antibacterial activity of the organic and chloroformic extracts of Artemisia annua against the tested microorganisms. Presence of tannins, saponins, alkaloids, amino acids, phenolic compounds, quinines and terpenoids were identified in the composition of the obtained extract using mass gas-chromatograph. The best result for the minimum inhibitory Concentration and minimum bactericidal concentration was reported for the 32 mg/ml of chloroformic extract.
Conclusion: The results indicate the fact that the extracts and essential oils of the plants can be useful as medicinal or preservatives composition6T.6T

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