Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells

Ali Dehghanifard; Saied Kaviani; Mehrdad Noruzinia; Masoud Soleimani; Saeid Abroun; Abbas Hajifathali; Ali Akbar Pourfathollah; Yousef Mortazavi; Mohammad Ahmadvand; Maryam Mahmoodinia-Meymand; Zeinab Kaviani; Majid Farshdousti-Hagh; Najmaldin Saki

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Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells

Author(s):

Ali Dehghanifard¹,

Saied Kaviani^1,*,

Mehrdad Noruzinia²,

Masoud Soleimani¹,

Saeid Abroun¹,

Abbas Hajifathali³,

Ali Akbar Pourfathollah⁴,

Yousef Mortazavi⁵,

Mohammad Ahmadvand⁶,

Maryam Mahmoodinia-Meymand⁷,

Zeinab Kaviani⁸,

Majid Farshdousti-Hagh⁹,

Najmaldin Saki¹⁰

1Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3Department of Hematology and Oncology, School of Medical Sciences, Shahid Beheshti University, Tehran, Iran

4Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

5Department of Pathology, Zanjan University of Medical Sciences, Zanjan, Iran

6Department of Laboratory Sciences, Zahedan University of Medical Sciences, Zahedan, Iran

7Department of Biology, Stem cell Research Center, Sarem Hospital, Tehran, Iran

8Department of Internal Medicine, Alborz University of Medical Sciences, Karaj, Iran

9Laboratory of Hematology and Blood Banking, Tabriz University of Medical Sciences, Tabriz, Iran

10Thalassemia and Hemoglobinopathy Research Center, Jundi Shapur University of Medical Sciences, Ahvaz, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 14, issue 7; e93309

Published online:Dec 19, 2011

Article type:Research Article

Received:Jun 26, 2011

Accepted:Aug 29, 2011

How to Cite:Ali DehghanifardSaied KavianiMehrdad NoruziniaMasoud SoleimaniSaeid AbrounAbbas HajifathaliAli Akbar PourfathollahYousef MortazaviMohammad AhmadvandMaryam Mahmoodinia-MeymandZeinab KavianiMajid Farshdousti-HaghNajmaldin Sakiet al.Synergistic Effect of Sodium Butyrate and Thalidomide in the Induction of Fetal Hemoglobin Expression in Erythroid Progenitors Derived from Cord Blood CD133 + Cells.Zahedan J Res Med Sci.14(7):e93309.

Abstract

Background: The use of drugs with the ability to induce production of fetal hemoglobin as a novel therapeutic approach in treating β-Hemoglobinopathies is considered. γ-globin gene expression inducer drugs including sodium butyrate and thalidomide can reduce additional α-globin chains accumulation in erythroid precursors.
Materials and Methods: In this experimental study, MACS kit was used to isolate CD133+ cells of umbilical cord blood. Further, the effect of two drugs of thalidomide and sodium butyrate were separately and combined studied on the induction of quantitative expression of β-globin and γ-globin genes in erythroid precursor cells derived from CD133+ stem cells in-vitro. For this purpose, the technique SYBR green Real-time PCR was used.
Results: Flow cytometry results showed that approximately 95% of purified cells were CD133+. Real-time PCR results also showed the increased levels of γ-globin mRNA in the cell groups treated with thalidomide, sodium butyrate and combination of drugs as 2.6 and 1.2 and 3.5 times respectively, and for β-globin gene, it is respectively 1.4 and 1.3 and 1.6 times compared with the control group (p<0.05).
Conclusion: The study results showed that the mentioned drug combination can act as a pharmaceutical composition affecting the induction of fetal hemoglobin expression in erythroid precursor cells derived from CD133 + cells.

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