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Outline
Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans
Authors
1Assistant Professor, Clinical Biochemistry Department, Faculty of Medicine, Shahid Beheshti University of Medicine, Tehran, Iran, Andorra
2Professor, Medical Biotechnology Department, Medical Sconces Faculty, Tarbiat Modares University, Tehran, Iran, Andorra
3- Assistant Professor, Infectious Disease Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra
4Assistant Professor, Mycology Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra
*Corresponding Author: Corresponding author: Zohreh Farahnezhad, Assistant Professor, Mycology Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra Email: z.farahnezhad@yahoo.com
Published online:May 11, 2013
Article type:Research Article
Received:Dec 26, 2012
Accepted:Apr 11, 2013
How to Cite:Rajabibazl M, Rasaee MJ, Nouri Fard M, Farahnezhad Z. Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans. Ann Mil Health Sci Res. 2013;11(2):e65198. doi:
Abstract
Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method.
Highlights
References
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Copyright
© 2013, Annals of Military and Health Sciences Research. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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