Archives of Clinical Infectious Diseases
Said Maham1, 
Fatemeh Fallah1,*, Gita Eslami2, Sepideh Shamsafar1, Rahele Radmanesh1, Bita Pourkaveh2The plant materials play a major role in primary health care as therapeutic regimen in many developing countries. In the present study, the ethanol extracts of mentha spicata or spearmint (M. spicata) and mentha piperita or peppermint (M. piperita) have been used to inactive mycobacterium bovis (M. bovis) in comparison to isoniazid.
After collecting and identifying the herbs, their ethanolic extract was prepared using percolation method. The extracts of M. spicata and M. piperita with different dilutions; 0.39,0.78,1.56,3.12,6.25,12.5,25,50,100,200,400 mg/ml were provided. M. bovis strain 1173 P2 was used in this study. This microorganism was confirmed by acid-fast staining (Ziehl-Neelsen). The bacteria were incubated at 37 C for a long time by inoculation into Middle Brook broth (Difco). Biochemical tests such as niacin, nitrate and urease were performed to confirm the organism (e.g. Feingold)(1) Agar diffusion and MIC methods (McFarland standard method and diffusion disk) were used to determine the antimicrobial activity of ethanolic extracts and the inhibition zones formed on the media were measured with a transparent ruler in millimeters.
The in vitro antibacterial activities of ethanolic extracts showed 0.39 mg/ ml consistency of M. spicata and 100 mg/ml consistency of M. piperita as the least concentrations which inhibit growth of M. bovis in comparison with isoniazid.
According to our findings, extracts of M. spicata and M. piperita could be used as raw materials for phytotherapy because of their antibacterial activities against M. bovis as TB etiology.
© 2011, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Jahanpour S, Ghazisaidi K, Davoodi H, Mazandarani M, Samet M, et al. Antimicrobial Effects of Folk Medicinal Plants From the North of Iran Against Mycobacterium tuberculosis. Arch Pediatr Infect Dis. 2015;3(1 TB):e18098. doi: https://doi.org/10.5812/pedinfect.18098
Hasannejad Z, Dehghani F, Seyyednejad SM, Motamedi H. Investigation of Antibacterial Activity of Ethanolic and Methanolic Extracts of Mentha pulegium L.. Zahedan J Res Med Sci. 2014;16(10):. doi:
dos Santos Sales V, Monteiro ÁB, Delmondes GDA, do Nascimento EP, Sobreira Dantas Nóbrega de Figuêiredo FR, et al. Antiparasitic Activity and Essential Oil Chemical Analysis of the Piper Tuberculatum Jacq Fruit. Iran J Pharm Res. 2018;17(1):e124733. doi: https://doi.org/10.22037/ijpr.2018.2179
Nickavar B, Alinaghi A, Kamalinejad M. Evaluation of the Antioxidant Properties of Five Mentha Species. Iran J Pharm Res. 2022;7(3):e128592. doi: https://doi.org/10.22037/ijpr.2010.766
Mehrgan H, Mojab F, Pakdaman S, Poursaeed M. Antibacterial Activity of Thymus pubescens Methanolic Extract. Iran J Pharm Res. 2022;7(4):e128604. doi: https://doi.org/10.22037/ijpr.2010.778
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