Archives of Clinical Infectious Diseases
Infectious Diseases and Tropical Medicine Research Center, SBUMS
Authors
Escherichia coli (E. coli) species are able to produce extended-spectrum ?-lactamases (ESBLs) that cause high resistance to the beta-lactam antibiotics. Therefore, determining the antibiotic susceptibility patterns in resistant organisms is necessary for suitable therapeutic approaches.
Totally, 260 clinical isolates of E. coli were collected from hospitals in Tehran during April- 2006 to April 2007. All suspected isolates were screened by disk diffusion method and the production of ESBL genes was investigated by phenotypic confirmatory tests. Microbroth dilution method was applied to determine the MIC of ceftazidime. Subsequently, isolates showing MICCAZ?2?g/ml were subjected to PCR targeting blaTEM and blaSHV genes.
Forty-nine percent of isolates contained ESBLs, among which 73.6% and 85.6% were ceftazidime- and cefotaxime-resistant, respectively. Molecular analysis showed 11.2% and 46.4% of ESBL producing isolates contain blaSHV and blaTEM genes, respectively.
Results revealed high percentage of ESBL genes among the clinical isolates of E. coli. Since the ESBL genes were detected in resistant isolates, it's necessary to test all isolates showing reduced susceptibility to thirdgeneration cephalosporins. The isolation of patients infected with ESBL producing isolates can be useful in controlling associated outbreaks.
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Kalantar D, Mansouri S, . Emergence of multiple ?-lactamases produced by Escherichia coli clinical isolates from hospitalized patient in Kerman, Iran. Jundishapur J Microbiol. 2010;3(4):. doi:
Besharati Zadeh S, Shakib P, Zolfaghari MR, Farajzadeh Sheikh A. Prevalence of Escherichia coli and Klebsiella pneumoniae, Producing Extended-Spectrum Beta-Lactamase (ESBLs) from Clinical Specimen in Khuzestan, Iran. Gene Cell Tissue. 2021;8(3):e112377. doi: https://doi.org/10.5812/gct.112377
Najar Peerayeh S, Eslami M, Memariani M, Siadat SD. High Prevalence of blaCTX-M-1 Group Extended-Spectrum β-lactamase Genes in Escherichia coli Isolates From Tehran. Jundishapur J Microbiol. 2013;6(7):e6863. doi: https://doi.org/10.5812/jjm.6863
Moosavian M, Ahmadkhosravy N. Survey of CTX-M Gene Frequency in Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae Isolates Using the Combination Disk and PCR Methods in Ahvaz, Iran. Jundishapur J Microbiol. 2016;9(11):e40423. doi: https://doi.org/10.5812/jjm.40423
Safari M, Abtahi H, Shojapoor M, Akbari M, Poorbabayi A. Determination of the Pattern of Antibiotic Resistance and Investigation of Extended-Spectrum Beta-Lactamase Production of Enterobacteriaceae Isolates of Clinical Specimens. Zahedan J Res Med Sci. 2012;14(8):e93266. doi:
More by these authors
Last Update: 1 hour ago
Last Update: 1 hour ago
Last Update: 17 years ago
Last Update: 5 days ago
Last Update: 34 minutes ago
Last Update: 2 days ago
