Archives of Clinical Infectious Diseases
Infectious Diseases and Tropical Medicine Research Center, SBUMS
Image Credit:Arch Clin Infect Dis
Outline
Molecular Diagnosis of Tuberculosis: a New Primer Design
Authors
Abstract
Background: Nucleic acid based detection of Mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis.
Patients and methods: Fifty suspected cases were screened for M. tuberculosis by using a new primer sequence and comparing its results with the primer (IS6110) most widely used for tuberculosis diagnosis.
Results: The mean age of cases was 33.6±14.3 years. Twenty one cases (42%) were positive for M. tuberculosis. Maximum positivity was found in sputum (10/19) followed by menstrual blood (4/8), blood (5/15), ascitic fluid (1/2), and pleural fluid (1/5).
Conclusion: The new designed primer was reproducible and in complete concordance with the PCR-results obtained by the standard method using IS 6110 insertion sequence in all 50 cases in which both methods were used.
Highlights
References
- 1.The references are available in PDF file.
Copyright
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Comparison of Culture and PCR Methods for Diagnosis of Mycobacterium tuberculosis in Different Clinical Specimens
Gholoobi A, Masoudi-Kazemabad A, Meshkat M, Meshkat Z. Comparison of Culture and PCR Methods for Diagnosis of Mycobacterium tuberculosis in Different Clinical Specimens. Jundishapur J Microbiol. 2014;7(2):e8939. doi: https://doi.org/10.5812/jjm.8939
Comparison of Culture and Microscopic Methods by PCR for Detection of Mycobacterium Tuberculosis in Sputum
Khorshidi A, Rohani M, Moniri R, Torfeh M. Comparison of Culture and Microscopic Methods by PCR for Detection of Mycobacterium Tuberculosis in Sputum. Arch Clin Infect Dis. 2009;4(4):. doi:
Determining the Diagnostic Value of Mycobacterium Tuberculosis DNA in the Differentiation of Blood Samples of Patients with Active Pulmonary Tuberculosis and Healthy Controls Using Polymerase Chain Reaction
Niazi A, Moulaei N, Shahriar M, karimian R, Peykfalak F. Determining the Diagnostic Value of Mycobacterium Tuberculosis DNA in the Differentiation of Blood Samples of Patients with Active Pulmonary Tuberculosis and Healthy Controls Using Polymerase Chain Reaction. Zahedan J Res Med Sci. 2013;15(10):e92829. doi:
Urine PCR Evaluation to Diagnose Pulmonary Tuberculosis
Heydari AA, Movahhede Danesh MR, Ghazvini K. Urine PCR Evaluation to Diagnose Pulmonary Tuberculosis. Jundishapur J Microbiol. 2014;7(3):e9311. doi: https://doi.org/10.5812/jjm.9311
Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction
Jobayer M, Shamsuzzaman S, Zulfiquer Mamun K. Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction. Arch Clin Infect Dis. 2014;9(2):20694. doi: https://doi.org/10.5812/archcid.20694
- Scopus by DOI: 0
Last Update: 1 month ago
- Scopus by Title: 4
Last Update: 1 month ago
- Scopus by Title (Ref): 4
Last Update: 1 month ago
- CrossRef: 0
Last Update: 5 days ago
- OpenAlex: 0
Last Update: 1 hour ago
- OpenCitations: 0
Last Update: 2 days ago