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Background:
Tuberculosis (TB) is a curable infectious disease yet the leading cause of death worldwide and in Bangladesh it is responsible for 7% of total deaths every year.
Archives of Clinical Infectious Diseases
Infectious Diseases and Tropical Medicine Research Center, SBUMS
Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction
Author(s):
1Department of Microbiology, Dhaka Medical College, Dhaka, Bangladesh
Published online:Jan 24, 2014
Article type:Research Article
Received:Sep 15, 2013
Accepted:Dec 04, 2013
How to Cite:Jobayer M, Shamsuzzaman S, Zulfiquer Mamun K. Rapid Diagnosis of Pulmonary Tuberculosis From Sputum by Polymerase Chain Reaction. Arch Clin Infect Dis. 2014;9(2):20694. doi: https://doi.org/10.5812/archcid.20694
Abstract
Objectives:
This cross sectional study was conducted to isolate and identify
Patients and Methods:
A total of 150 clinically suspected pulmonary TB patients (male/female: 97/53; age: 31.9 9.8 years) were enrolled in this study. Freshly passed single morning sputum was collected from each patient and diagnostic efficacy of PCR was compared with staining and culture methods.
Results:
Among 150 sputum samples, 25 (16.7%) were positive by Ziehl-Neelsen (ZN) stain, 37 (24.7%) yielded growth in Lowenstein-Jensen (LJ) media and 45 (30%) were positive by PCR. Of the 37 isolated
Conclusions:
PCR was the most sensitive, accurate and rapid method for diagnosis of TB. It was concluded that, for the diagnosis of pulmonary tuberculosis, PCR directly from sputum may be recommended in Bangladesh, especially in suspected tuberculosis patients who remain negative by conventional methods.
Copyright
© 2014, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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