Rho GDP Protein Expression Change and Investigation of ItsEffect on GDP/GTP Cycle in Human Brain Oligodendroglioma

Background: Oligodendrogliomas are sub-types of Gliomas with an enriched net of branching capillary. A vast amount of Oligodendroglial (approximately 60-90%) demonstrates a loss of 1p and 19q chromosomes, and their response to radio and chemotherapy treatments are considerably more favorable and less aggressive. Rho GDP inhibitor is a protein which controls the activity and distribution of GTPase in cells. In cancer cells with 1pLOH deletion, Rho GDP inhibitor protein can be anti-apoptotic. Here understanding of molecular diagnosis of Oligodendroglioma tumors is investigated via proteomic tools.

Methods: Proteins of tumor and normal brain tissues are extracted and separated by Two-Dimensional Gel (2DG) Electrophoresis method and the spots were then analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by pI, molecular weights and data banks.

Results: The 2D gels of normal and patient were provided and compared. As a result,high resolution analysis showed that there are totally 1328 spots in the gel. Bioinformatic analysis of the gels revealed that Rho GDI is one of the downregulated proteins.

Conclusion: It was revealed that Rho GDP inhibitor deactivates GTPProtein complex through disintegration of GTP from Rho proteins, leading to prevention of Rho GDP to return to its cycling.