High-Performance Liquid Chromatography Determination of Methotrexate in Plasma
Authors
Abstract
This article describes a simple and fast high-performance liquid chromatography method for the determination of methotrexate (MTX) in plasma. Samples were collocted from children receiving high-dose MTX at shafa Hospital (Ahvaz University of Medical sciences, Ahvaz, iran) at various times after the end of eachinfusion. Plasma was deproteinized with trichloroacetic acid and the supernatant was injected into a 250×4.6 mm octadecylsilane column. Mobile phase was made of TRIS-phosphate buffer (pH 5.7): methanol: acetonitrile (82:11:7) with a flow rate of 1.8 ml/min. Ultraviolet detection was done at 313 nm and at ambient temperature. Para- aminoacetophenone was used as internal standard. Methotrexate and internal standard retention times were 4.4 and 6.5 minutes, respectively. Results showed that reproducibility (precision) of method within a day was 2.6 to 6 percent and between days was 5.5 to 9.5 percent. The recovery of the method was between 61.5 and 72.7 percent. The quantitation limit of the method for methotrexate was 0.1 μM. This method is suitable for quantitation of methotrexate after infusion of high doses of this drug and has good accuracy, precision and quantiatation limit.
Highlights
Copyright
© 2022, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Pharmacokinetics and Bioequivalence of Methotrexate in Human Plasma Studied by Liquid Chromatography-Mass Spectrometry (LC-MS)
Danafar H, Hamidi M. Pharmacokinetics and Bioequivalence of Methotrexate in Human Plasma Studied by Liquid Chromatography-Mass Spectrometry (LC-MS). Jundishapur J Nat Pharm Prod. 2016;11(2):e36758. doi: https://doi.org/10.17795/jjnpp-36758
Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection
Dasht Bozorg B, Goodarzi A, Fahimi F, Tabarsi P, Shahsavari N, et al. Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection. Iran J Pharm Res. 2019;18(4):e124712. doi: https://doi.org/10.22037/ijpr.2019.1100849
High Performance Liquid Chromatographic Estimation of Drotaverine Hydrochloride and Mefenamic Acid in Human Plasma
Dahivelkar PP, Bari SB, Bhoir S, Bhagwat AM. High Performance Liquid Chromatographic Estimation of Drotaverine Hydrochloride and Mefenamic Acid in Human Plasma. Iran J Pharm Res. 2022;8(3):e128639. doi: https://doi.org/10.22037/ijpr.2010.812
Determination of Methotrexate in Biological Fluids and a Parenteral Injection Using Terbium-Sensitized Method
Jouyban A, Shaghaghi M, L. Manzoori J, Soleymani J, JalilVaez-Gharamaleki J. Determination of Methotrexate in Biological Fluids and a Parenteral Injection Using Terbium-Sensitized Method. Iran J Pharm Res. 2011;10(4):e125995. doi: https://doi.org/10.22037/ijpr.2011.990
Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization
Majnooni M, Jalili R, Mohammadi B, Miraghaee S, Bahrami MT, et al. Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography UV detection after pre column derivatization. J Rep Pharm Sci. 2014;3(2):e147711. doi:
- Scopus by DOI: 22
Last Update: 3 weeks ago
- Scopus by Title: 22
Last Update: 3 weeks ago
- Scopus by Title (Ref): 2
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 6 days ago
- OpenAlex: 27
Last Update: 7 hours ago
- OpenCitations: 1
Last Update: 4 days ago