Evaluation of potency of measles vaccine used in Iran: comparison of WHO and NIBSC method in cell culture

Shohreh Tavajohi; Hossein Rastegar; Seyed Nasser Ostad; Seyed Mehdi Rezayat; Mohammad  Hossein Ghahremani

doi:10.22037/ijpr.2010.631

IJ Pharmaceutical Research

Current Issue All Issues In Press Accepted Manuscripts

Journal Information Editors & Boards Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Reviewer and AE Registration Form Support Contact Us

APC

Authors Guide Submit Manuscript

https://doi.org/10.22037/ijpr.2010.631

Evaluation of potency of measles vaccine used in Iran: comparison of WHO and NIBSC method in cell culture

Author(s):

Shohreh Tavajohi^{1, 2},

Hossein Rastegar¹,

Seyed Nasser Ostad³,

Seyed Mehdi Rezayat²,

Mohammad Hossein Ghahremani^3,*

1Food and Drug Control Lab, Ministry of Health and Medical Education, Tehran, Iran

2Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

3Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

IJ Pharmaceutical Research:Vol. 4, issue 3; 155-160

Published online:Nov 20, 2010

Article type:Research Article

Received:May 20, 2004

Accepted:Sep 20, 2005

How to Cite:Tavajohi S, Rastegar H, Ostad SN, Rezayat SM, Ghahremani MH. Evaluation of potency of measles vaccine used in Iran: comparison of WHO and NIBSC method in cell culture. Iran J Pharm Res. 2022;4(3):e128239. doi: https://doi.org/10.22037/ijpr.2010.631

Abstract

Measles has been a major cause of illness and death in children and vaccination against the disease is part of the WHO global immunization program. A suitable vaccine should create maximum immune response against the pathogen and must be safe for the user. Thus, after production, vaccines must be analyzed and controlled by the producer and confirm by relevant governmental organizations. The Food and Drug Control Lab (FDCL), Ministry of Health, is the secondary control center on potency of vaccines in Iran. In this study, we have set up the WHO and NIBSC methods in FDCL and compare these methods on determining the potency of measles vaccine.

Measles vaccines were obtained from Razi Institute Iran. Nine dilutions of vaccine (10^-1 to 10^-5) in 0.5 log interval were mixed with Vero cell suspension and seeded. In WHO method, the cells were incubated at 36°C for 10 days, during which the cells were checked for cytopatic changes everyday. To set up the assay, we tested the vaccine dilution with four different cell suspensions (2×10⁵-5×10⁴/well) and four different concentration of serum (2.5-10%). Based on our results, in the assays, 5% serum and 1×10⁵ cells were used. The potency assay was performed with six different vaccines produced in one batch and the mean potency for Measles was 10^{4.32 ± 0.24}CCID₅₀/vial for a ten-dose vial. In NIBSC method following seeding of Vero cells, the medium was removed after 3 hours and overlay was added. Then the plates were incubated at 35°C for 10 days. After incubation period, the overlay was removed, the plaques were stained with methyl violet and counted. This assay was repeated three times and the mean of the results was 5.83 ± 0.03 log₁₀ PFU/dose.

In this study, we have set up the WHO and NIBSC methods and results indicated that the potency of the vaccine is in acceptable range in either method. Furthermore, the WHO method is simple and less time consuming compared to NIBSC which is complicated and requires more effort to produce reproducible results.

Keywords

Measles Vaccines in Iran: A 50-Year Review of Vaccine Development, Production and Effectiveness (1967 - 2017)

Abbas Shafyi,

Ashraf Mohammadi

Shafyi A, Mohammadi A. Measles Vaccines in Iran: A 50-Year Review of Vaccine Development, Production and Effectiveness (1967 - 2017). Jundishapur J Microbiol. 2018;11(5):e60725. doi: https://doi.org/10.5812/jjm.60725

1

Dec

2013

Post-Vaccination Immunity Against Measles in Under Twenty-Five-Year-Old Population of Ahvaz, Southwest of Iran

Abdolhussein Shakurnia,

Seyed Mohammad Alavi,

Reza Norouzirad,

Amir Arsalan Sarajian,

Ghodratollah Shakerinejad

Shakurnia A, Alavi SM, Norouzirad R, Sarajian AA, Shakerinejad G. Post-Vaccination Immunity Against Measles in Under Twenty-Five-Year-Old Population of Ahvaz, Southwest of Iran. Jundishapur J Microbiol. 2013;6(10):e7707. doi: https://doi.org/10.5812/jjm.7707

15

Jun

2004

Epidemiologic survey of documented measles outbreak in Tehran

1 Mokhtari,

M Alavi Moghaddam,

D Yadegari,

Masoud Salehi,

F Shahim,

Iraj Zareban

Mokhtari 1, Alavi Moghaddam M, Yadegari D, Salehi M, Shahim F, et al. Epidemiologic survey of documented measles outbreak in Tehran. Zahedan J Res Med Sci. 2004;6(2):e95020. doi:

9

Jul

2019

Assessment of Routine Measles Vaccine Effectiveness Among Children Referring to Tertiary Fever Hospital in Egypt

John Rene Labib,

Eman Hany Elsebaie,

Shaimaa A.M. Abd El Fatah,

Silvia Farouk Shalaby,

Engy El Khateeb

Labib JR, Elsebaie EH, Abd El Fatah SA, Shalaby SF, El Khateeb E. Assessment of Routine Measles Vaccine Effectiveness Among Children Referring to Tertiary Fever Hospital in Egypt. Arch Pediatr Infect Dis. 2019;7(3):e90407. doi: https://doi.org/10.5812/pedinfect.90407

31

Jul

2007

Evaluation of Primary Failure of New Measles Vaccine and Secondary Failure of Previous Measles Vaccine After Mass Vaccination in Military Cadets in Iran

Gholamali Ghorbani,

Kazem Ahmadi,

Hamidreza Ghadimi

Ghorbani G, Ahmadi K, Ghadimi H. Evaluation of Primary Failure of New Measles Vaccine and Secondary Failure of Previous Measles Vaccine After Mass Vaccination in Military Cadets in Iran. Arch Clin Infect Dis. 2007;2(3):. doi:

Import into EndNote Import into BibTex

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 2
Last Update: 1 week ago
Scopus by Title: 2
Last Update: 1 week ago
Scopus by Title (Ref): 0
Last Update: 1 week ago
CrossRef: 0
Last Update: 3 days ago

Metrics

Licensing and reuse information

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Shohreh Tavajohi:[PubMed][Scholar]
Hossein Rastegar:[PubMed][Scholar]
Seyed Nasser Ostad:[PubMed][Scholar]
Seyed Mehdi Rezayat:[PubMed][Scholar]
Mohammad Hossein Ghahremani:[PubMed][Scholar]

Outlines

Evaluation of potency of measles vaccine used in Iran: comparison of WHO and NIBSC method in cell culture

Abstract

Similar Articles

Measles Vaccines in Iran: A 50-Year Review of Vaccine Development, Production and Effectiveness (1967 - 2017)

Post-Vaccination Immunity Against Measles in Under Twenty-Five-Year-Old Population of Ahvaz, Southwest of Iran

Epidemiologic survey of documented measles outbreak in Tehran

Assessment of Routine Measles Vaccine Effectiveness Among Children Referring to Tertiary Fever Hospital in Egypt

Evaluation of Primary Failure of New Measles Vaccine and Secondary Failure of Previous Measles Vaccine After Mass Vaccination in Military Cadets in Iran

Crossmark

Checking

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 1 week ago

Last Update: 3 days ago