Critical role of GSH in Sulfur Mustard-induced Oxidative Stress and Cytotoxicity in Human Skin Fibroblast Cell Line
Authors
Abstract
In this study the role of glutathione (GSH) in sulfur mustard -induced oxidative stress and cytotoxicity, in human skin fibroblast cell line (HF2FF) was evaluated. Sulfur mustard-induced superoxide radical and hydrogen peroxide formation were evaluated by determination of superoxide dismutase and catalase activity in cell lysate. The cytotoxicity of sulfur mustard was estimated by lactate dehydrogenase leakage. The intracellular GSH content was modulated by N-acetylcysteine (NAC), a GSH precursor, and buthionine sulfoximine (BSO), a specific GSH synthesis inhibitor. It was found that sulfur mustard exposure led to a dose-and time-dependent decrease in GSH content in HF2FF cells. NAC increased intracellular GSH level and protected the cells against sulfur mustard-induced reactive oxygen species formation and lactate dehydrogenase leakage. In contrast, buthionine sulfoximine pretreatment depleted cellular GSH and enhanced the susceptibility of HF2FF to the cytotoxic effects of sulfur mustard. These results indicated that GSH plays a critical role in protecting HF2FF cell line against sulfur mustar-induced cell injury, most probably through its antioxidant activity.
Highlights
Copyright
© 2022, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
Extra cellular Superoxide Dismutase activity in lung injuries due to Sulfur Mustard
Shohrati M, Ghanei M, Shamspour N, Jafari M, Norozi Abdi Khasmakhi M. Extra cellular Superoxide Dismutase activity in lung injuries due to Sulfur Mustard. J Inflamm Dis. 2024;12(4):e155430. doi:
Activation of Nrf2-Antioxidant Response Element Mediated Glutamate Cysteine Ligase Expression in Hepatoma Cell line by Homocysteine
Mani M, Khaghani S, Gol Mohammadi T, Zamani Z, Azadmanesh K, et al. Activation of Nrf2-Antioxidant Response Element Mediated Glutamate Cysteine Ligase Expression in Hepatoma Cell line by Homocysteine. Hepat Mon. 2013;13(5):e8394. doi: https://doi.org/10.5812/hepatmon.8394
Two Lung Cancer Development-Related Genes, Forkhead Box M1 (FOXM1) and Apolipoprotein E (APOE), are overexpressed in Bronchial of Patients after Long-Term Exposure to Sulfur Mustard
Tahmasbpour E, Ghanei M, Panahi Y. Two Lung Cancer Development-Related Genes, Forkhead Box M1 (FOXM1) and Apolipoprotein E (APOE), are overexpressed in Bronchial of Patients after Long-Term Exposure to Sulfur Mustard. Iran J Pharm Res. 2017;16(4):e125063. doi: https://doi.org/10.22037/ijpr.2017.2138
Study of The Effects of N-Acetylcysteine on Oxidative Stress Status of Patients on Maintenance-Hemodialysis Undergoing Cadaveric Kidney Transplantation
Modarresi A, Ziaie S, Salamzadeh J, Sahraei Z, Nafar M, et al. Study of The Effects of N-Acetylcysteine on Oxidative Stress Status of Patients on Maintenance-Hemodialysis Undergoing Cadaveric Kidney Transplantation. Iran J Pharm Res. 2017;16(4):e125037. doi: https://doi.org/10.22037/ijpr.2017.2139
Effects of Mustard Gas on Veterans: A Review
Ghaednia Jahromy M, Mohsenian MS, Rezagholizamenjany M, Ansari I, Mirzadeh SM, et al. Effects of Mustard Gas on Veterans: A Review. Ann Mil Health Sci Res. 2017;15(2):e62051. doi: https://doi.org/10.5812/amh.62051
More by these authors
- Scopus by DOI: 0
Last Update: 3 weeks ago
- Scopus by Title: 10
Last Update: 3 weeks ago
- Scopus by Title (Ref): 0
Last Update: 3 weeks ago
- CrossRef: 0
Last Update: 1 hour ago
- OpenAlex: 8
Last Update: 12 hours ago
- OpenCitations: 0
Last Update: 4 days ago