Expression Profiles of P53, Caspase-3 And Bcl-2 in Patients Undergoing Congenital Heart Corrective surgery: Combined Effects Of Anesthesia And Surgery

Background:?Apoptosis is a physiological programmed cell death necessary for ??development and cellular homeostasis. Dysregulation of apoptosis pathways leads to a number of diseases such as cancer, autoimmune and immunodeficiency diseases, and neuro-degenerative disorders. ? Patients and methods: The current study included 56 patients (29 males and 27 females), undergoing corrective heart surgery operations, categorized into 3 groups: group A included 23 patients with atrial septal defect (ASD); group B included 15 patients with ventricular septal defect (VSD); and group C included 15 patients with fallot tetralogy. Biochemical assays of apoptotic (P53 ?and caspase 3) and antiapoptotic markers (Bcl-2)?using ?colorimetric and ELISA assay kits were performed to all included patients twice, preoperative and 24 hours postoperative. ?Results:?No statistical significant difference was found in the preoperative levels of Bcl-2, Caspase-3 and P53 between the three groups. While significant difference was found when comparing the preoperative and postoperative levels of the previous markers in the same group and between groups (p?0.05 for all). Conclusion: Both apoptotic and antiapoptotic pathways are activated during congenital heart corrective surgeries. Degree of markers increase is directly related to duration of anesthesia exposure. Key words:?P53; caspase-3; Bcl-2; congenital heart corrective surgery; Apotosis.