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Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Cloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli
Author(s):
1Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, [email protected], Iran
2Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Iran
*Corresponding Author: Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, [email protected], Iran. Email: [email protected]
Published online:Jan 31, 2009
Article type:Research Article
Received:Sep 01, 2008
Accepted:Mar 01, 2009
How to Cite:Galehdari H, Alaee S, Mirzaee M. Cloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli. Jundishapur J Microbiol. 2009;2(1):. doi:
Abstract
The Azotobacter vinelandii is an aerobic, nitrogen-fixing bacterium with the ability to accumulate polyesters such as poly (3-hydroxybutyrate), a particular group of the polyhydroxyalkanoates (pha), when will be cultivated on several carbon sources, including sucrose. Although the capacity of Azotobacter strains to accumulate polyesters is well known, and the genes responsible for their synthesis have recently been identified. However, high production costs have hindered the use of polyhydroxyalkanoates as bioplastics, since their final price is considerably high. The phb gene clusters including phbBAC genes can alternatively be cloned and expressed in the Escherichia coli with the goal to produce high cell density cultivation. We amplified separately the phbB, phbA and phbC genes from extracted Azotobacter genome with the designed primers and could clone the fragments in the cloning vector pCR 2.1- TOPO with the subsequent transformation of E. coli strain DH5α.
Copyright
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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