Fulltext
Full text is available in PDF
Jundishapur Journal of Microbiology
Ahvaz Jundishapur University of Medical Sciences
Molecular Characterization of Leishmania Infection in Sand flies From Sistan Va Baluchistan Province, Southeastern Iran
Author(s):
1Department of Medical Entomology and Vector Control, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2Department of Medical Parasitology, Pasteur Institute of Iran, Tehran, IR Iran
3Department of Medical Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
4Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
*Corresponding Author: Hamid Kassiri, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran. Tel: +98-6113738269, Fax: +98-6113738282, E-mail: Email: [email protected]
Published online:Apr 30, 2012
Article type:Research Article
Received:Jul 01, 2011
Accepted:Nov 25, 2011
How to Cite:Kassiri H, Naddaf SR, Mohebali M, Javadian E. Molecular Characterization of Leishmania Infection in Sand flies From Sistan Va Baluchistan Province, Southeastern Iran. Jundishapur J Microbiol. 2012;5(2):. doi: https://doi.org/10.5812/jjm.3523
Abstract
Background: Cutaneous leishmaniasis (CL) is a zoonotic disease that is caused by various species of the genus Leishmania. The disease is considered a major health problem in different areas of Iran and is an endemic disease in rural areas of Mirjaveh, Chabahar, and Konarak Counties, Sistan Va Baluchistan Province.
Objectives: The aim of this study was to identify Leishmania species that was isolated from potential sand fly vectors by molecular analysis in Chabahar County.
Materials and Methods: To collect Sand flies, sticky traps were placed at the entrance of rodents burrows in Dashtiyari division of Chabahar County, where CL is endemic. Freshly collected Sand flies were identified with regard to species, dissected in normal saline using binocular, and examined for leptomonads under a microscope. Leptomonads from the Sand flies were used to inoculate the base of Balb/c mice tails subcutaneously; after an incubation period and the development of lesions, the parasites were transferred to NNN + LIT medium culture. The harvested Leishmania parasites were subjected to DNA extraction and analyzed by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR).
Results: DNA from Leishmania species from Phlebotomus papatasi and P. salehi Sand flies produced distinctive patterns of bands of L. major with all primers. However, the products at approximately 2100 bp and 800 bp that were amplified with primer 329 were stable and reproducible in all assays. This is the first report on the isolation and identification of L. major in P. salehi from Iran and P. papatasi from Sistan va Baluchistan.
Conclusions: The study shows that P. papatasi and P. salehi Sand flies play a major role in the maintenance and transmission of disease to humans in this area.
Implication for health policy/practice/research/medical education:
Identification of P. papatasi and P. salehi Sand flies as the insect vectors as well as the Leishmania species they harbor would be the key elements in understanding the epidemiology of leishmaniasis and its control in Chabahar County.
Copyright © 2012 Kowsar Corp. All rights reserved.
Copyright
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
10
Aug
2013
Isolation and Identification of Leishmania Species From Sandflies and Rodents Collected From Roffaye District, Khuzestan Province, Southwest of Iran.
Babak Vazirianzadeh,
Jasem Saki,
Elham Jahanifard,
Mehdi Zarean,
Kamyar Amraee,
Sharokh Navid Pour
Vazirianzadeh B, Saki J, Jahanifard E, Zarean M, Amraee K, et al. Isolation and Identification of Leishmania Species From Sandflies and Rodents Collected From Roffaye District, Khuzestan Province, Southwest of Iran.. Jundishapur J Microbiol. 2013;6(6):e10025. doi: https://doi.org/10.5812/jjm.10025
31
Jul
2011
Bionomics of Phlebotomine Sand Flies (Diptera: Psychodidae) as Vectors of Leishmaniasis in the County of Iranshahr, Sistan-Baluchistan Province, Southeast of Iran
Kassiri Hamid,
Javadian Ezat Aldin,
Sharififard Mona
Hamid K, Ezat Aldin J, Mona S. Bionomics of Phlebotomine Sand Flies (Diptera: Psychodidae) as Vectors of Leishmaniasis in the County of Iranshahr, Sistan-Baluchistan Province, Southeast of Iran. Arch Clin Infect Dis. 2011;6(3):. doi:
21
Aug
2012
Phlebotominae Sandflies Fauna (Diptera: Psychodidae) in Hamadan, Iran
Mansour Nazari,
Amir Hosein Zahirnia
Nazari M, Zahirnia AH. Phlebotominae Sandflies Fauna (Diptera: Psychodidae) in Hamadan, Iran. Zahedan J Res Med Sci. 2012;14(8):e93262. doi:
15
Jul
2012
Some Ecological Characteristics of Phlebotomine sandflies in a Focus of Cutaneous Leishmaniasis, Chabahar, Iran
Hamid Kassiri,
Ezatodin Javadian
Kassiri H, Javadian E. Some Ecological Characteristics of Phlebotomine sandflies in a Focus of Cutaneous Leishmaniasis, Chabahar, Iran. Zahedan J Res Med Sci. 2012;14(8):e93263. doi:
1
Aug
2013
Molecular Identification of Leishmania Species Isolated From Human Cutaneous Leishmaniasis in Poledokhtar District, Lorestan Province, Iran
Elahe Beiranvand,
Mohsen Kalantari,
Hasan Ali Rastgar,
Kamyar Amraee
Beiranvand E, Kalantari M, Rastgar HA, Amraee K. Molecular Identification of Leishmania Species Isolated From Human Cutaneous Leishmaniasis in Poledokhtar District, Lorestan Province, Iran. Jundishapur J Microbiol. 2013;6(6):e8103. doi: https://doi.org/10.5812/jjm.8103
Download PDF595.13 KB
Crossmark
Checking
Comments
Number of Comments:0Cited by
- Scopus by DOI: 16
Last Update: 2 weeks ago
- Scopus by Title: 16
Last Update: 2 weeks ago
- Scopus by Title (Ref): 16
Last Update: 2 weeks ago
- CrossRef: 14
Last Update: 5 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Search Relations
Author(s):
Related Articles