Multi-Drug Resistant Acinetobacter-Derived Cephalosporinase and OXAsetC Genes in Clinical Specimens of Acinetobacter spp. Isolated From Teaching Hospital

How to Cite:Farahani R, Moniri R, Dastehgoli Farahani K, Farahani R. Multi-Drug Resistant Acinetobacter-Derived Cephalosporinase and OXAsetC Genes in Clinical Specimens of Acinetobacter spp. Isolated From Teaching Hospital. Jundishapur J Microbiol. 2013;6(2):. doi: https://doi.org/10.5812/jjm.5059

Abstract

Hospital-acquired infections caused by multi-drug resistant Acinetobacter spp. are often extremely difficult to treatand this has proved to be a serious problem worldwide.

The aim of this study was to determine the incidence rates and distribution patterns of multi-drug resistant (MDR) Acinetobacter spp. strains and the occurrence of Acinetobacter-derived cephalosporinase (ADC-7) and OXA-type carbapenemases (OXAsetC genes) in clinical specimens in the Beheshti Teaching Hospital in Kashan, Iran.

This descriptive study was carried out on sixty isolates of Acinetobacter spp. and clinical samples collected from patients. The level of antibiotic resistance was determined by the disc diffusion method and the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) procedure. Polymerase chain reaction (PCR) amplification of the genetic determinants of resistance was also determined.

The resistance rates were; amikacin (80%), tobramycin (68.3%), ceftazidime (60%), ciprofloxacin (55%), piperacillin/tazobactam (51.7%), doxycycline (50%), SXT/TMP (sulfamethoxazole / trimethoprim)(48.3%), levofloxacin (43.3%), gentamicin (40%), imipenem (25%), and sulbactam/ampicillin (20%). The frequency of MDR Acinetobacter spp. strains isolated was found to be 56.7%. These isolates were most sensitive to imipenem followed by ampicillin/sulbactam and gentamicin. The prevalence of genes for ADC-7 and OXAsetC in the Acinetobacter spp. was; 34 (56.7%) and 32 (53.3%), respectively. The positive percentages of MDR isolates for ADC-7 were 82.4% and for OXAsetC they were 73.5%.

Our phenotypic analysis demonstrated that Acinetobacter spp. isolates were resistant to most clinically significant antibiotic classes. This is the first report concerning Acinetobacter-derived cephalosporinase, blaADC, enzymes in Acinetobacter spp. isolates from Iran.

Keywords

Acinetobacter spp.

Acinetobacter-Derived Cephalosporinase

OXA-Type Carbapenemases

Antibiotic Susceptibility

Iran

Fulltext

Full text is available in PDF

Import into EndNote Import into BibTex

Crossmark

Checking

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 8
Last Update: 1 month ago
Scopus by Title: 8
Last Update: 1 month ago
Scopus by Title (Ref): 8
Last Update: 1 month ago
CrossRef: 3
Last Update: 1 hour ago

Metrics

Licensing and reuse information

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Reza Farahani:[PubMed][Scholar]
Rezvan Moniri:[PubMed][Scholar]
Kamran Dastehgoli Farahani:[PubMed][Scholar]
Reza Farahani:[PubMed][Scholar]

Ahvaz Jundishapur University of Medical Sciences

Outlines

Multi-Drug Resistant Acinetobacter-Derived Cephalosporinase and OXAsetC Genes in Clinical Specimens of Acinetobacter spp. Isolated From Teaching Hospital

Abstract

Fulltext

Crossmark

Checking

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 1 hour ago